DAP-3 Antibody (F-12): sc-373911
- DAP-3 Antibody (F-12) is a mouse monoclonal IgG1 κ DAP-3 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 63-95 within an internal region of DAP-3 of human origin
- DAP-3 Antibody (F-12) is recommended for detection of DAP-3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DAP-3 Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DAP-3 Antibody (F-12) for WB applications. These reagents are now offered in bundles with DAP-3 Antibody (F-12) (see ordering information below).
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DAP-3 Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects DAP-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP-3 Antibody (F-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Death-associated protein 3 (DAP-3) is a nucleotide-binding protein characterized by a potential P-loop motif, playing a crucial role as a positive mediator of programmed cell death. DAP-3 is required for apoptosis induction, with the full-length protein being essential for activity. DAP-3 operates downstream of receptor signaling complexes, and pro-apoptotic effects are contingent upon caspase activity. DAP-3 interacts with the glucocorticoid receptor (GR), specifically within the amino-terminal region, acting in a dominant-negative manner to protect cells from apoptosis. This interaction highlights DAP-3′s importance in cellular survival mechanisms. DAP-3 is conserved both functionally and structurally, exhibiting ubiquitous expression in highly proliferative epithelial compartments across various tissues. DAP-3 maintains mitochondrial localization during apoptosis induction, underscoring a unique role in cell death pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DAP-3 Antibody (F-12) References:
- DAP kinase and DAP-3: novel positive mediators of apoptosis. | Kimchi, A. 1999. Ann Rheum Dis. 58 Suppl 1: I14-9. PMID: 10577968
- The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the glucocorticoid receptor and affects the receptor function. | Hulkko, SM., et al. 2000. Biochem J. 349 Pt 3: 885-93. PMID: 10903152
- The apoptosis mediator mDAP-3 is a novel member of a conserved family of mitochondrial proteins. | Berger, T., et al. 2000. J Cell Sci. 113 (Pt 20): 3603-12. PMID: 11017876
- Death-associated protein 3 (Dap-3) is overexpressed in invasive glioblastoma cells in vivo and in glioma cell lines with induced motility phenotype in vitro. | Mariani, L., et al. 2001. Clin Cancer Res. 7: 2480-9. PMID: 11489830
- Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. | Kissil, JL., et al. 1995. J Biol Chem. 270: 27932-6. PMID: 7499268
- Structure-function analysis of an evolutionary conserved protein, DAP3, which mediates TNF-alpha- and Fas-induced cell death. | Kissil, JL., et al. 1999. EMBO J. 18: 353-62. PMID: 9889192
- Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions. | Levy-Strumpf, N. and Kimchi, A. 1998. Oncogene. 17: 3331-40. PMID: 9916995
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAP-3 Antibody (F-12) | sc-373911 | 200 µg/ml | $322.00 | |||
DAP-3 Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-525927 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAP-3 Antibody (F-12): m-IgG1 BP-HRP Bundle | sc-533464 | 200 µg Ab; 20 µg BP | $361.00 | |||
DAP-3 Antibody (F-12) AC | sc-373911 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DAP-3 Antibody (F-12) HRP | sc-373911 HRP | 200 µg/ml | $322.00 | |||
DAP-3 Antibody (F-12) FITC | sc-373911 FITC | 200 µg/ml | $336.00 | |||
DAP-3 Antibody (F-12) PE | sc-373911 PE | 200 µg/ml | $349.00 | |||
DAP-3 Antibody (F-12) Alexa Fluor® 488 | sc-373911 AF488 | 200 µg/ml | $364.00 | |||
DAP-3 Antibody (F-12) Alexa Fluor® 546 | sc-373911 AF546 | 200 µg/ml | $364.00 | |||
DAP-3 Antibody (F-12) Alexa Fluor® 594 | sc-373911 AF594 | 200 µg/ml | $364.00 | |||
DAP-3 Antibody (F-12) Alexa Fluor® 647 | sc-373911 AF647 | 200 µg/ml | $364.00 | |||
DAP-3 Antibody (F-12) Alexa Fluor® 680 | sc-373911 AF680 | 200 µg/ml | $364.00 | |||
DAP-3 Antibody (F-12) Alexa Fluor® 790 | sc-373911 AF790 | 200 µg/ml | $364.00 | |||
DAP-3 (F-12) Neutralizing Peptide | sc-373911 P | 100 µg/0.5 ml | $69.00 |