DAP-3 Antibody (D-4): sc-398416

DAP-3 Antibody (D-4) is a mouse monoclonal IgG1 antibody that detects DAP-3 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP-3 is a crucial nucleotide-binding protein that plays a significant role in the regulation of programmed cell death, making DAP-3 essential for maintaining cellular homeostasis and tissue integrity. DAP-3 (D-4) antibody recognizes a protein known for mediating apoptosis, with its full-length form being necessary for the induction of this process. DAP-3 operates downstream of receptor signaling complexes, and DAP-3′s pro-apoptotic effects are contingent upon the activity of caspases, which are key enzymes in the apoptosis pathway. Notably, DAP-3 interacts with the glucocorticoid receptor, specifically through its amino-terminal region, which acts in a dominant-negative manner to shield cells from undergoing apoptosis. This interaction highlights DAP-3′s dual role in both promoting and inhibiting cell death, depending on the cellular context. DAP-3 is conserved across species at both functional and structural levels, and DAP-3 is widely expressed in highly proliferative epithelial tissues, underscoring DAP-3′s importance in various biological processes. Uniquely, DAP-3 maintains mitochondrial localization even during apoptosis, distinguishing DAP-3 from many other apoptotic regulators and emphasizing DAP-3′s critical function in cellular responses to stress.

The preferred DAP-3 antibody is DAP-3 Antibody (F-12): sc-373911.