Dab1 Antibody (G-5): sc-271136

Dab1 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dab1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Dab1 antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dab1, a homolog of the Drosophila Disabled protein, plays a crucial role as an adaptor protein in neural development, particularly during the rapid expansion of the developing nervous system. Located primarily in the cytoplasm, Dab1 undergoes tyrosine phosphorylation, which is essential for its function in signaling pathways that guide the positioning of neurons. This phosphorylation allows Dab1 to interact with other proteins, notably through its PTB-like domain, facilitating its role in the Reelin signaling pathway. Reelin, a secreted protein, is vital for the proper migration and layering of neurons in the brain, and Dab1 acts downstream of Reelin, mediating its effects. Mutations in Dab1 can lead to significant brain abnormalities, mirroring the defects observed in Reelin mutants, underscoring the importance of Dab1 in maintaining neural architecture and function. Additionally, Dab1 has been shown to interact with the SH2 domains of Src, Fyn, and Abl, further highlighting its role in critical signaling networks that regulate neuronal development and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Dab1 Antibody (G-5) References:

1. Dab1 contributes differently to the morphogenesis of the hippocampal subdivisions.  |  Blume, M., et al. 2017. Dev Growth Differ. 59: 657-673. PMID: 28945921
2. Endothelial Dab1 signaling orchestrates neuro-glia-vessel communication in the central nervous system.  |  Segarra, M., et al. 2018. Science. 361: PMID: 30139844
3. Dab1-deficient deep layer neurons prevent Dab1-deficient superficial layer neurons from entering the cortical plate.  |  Yoshinaga, S., et al. 2022. Neurosci Res. 180: 23-35. PMID: 35364133
4. A neurodevelopmental epigenetic programme mediated by SMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastoma metastasis.  |  Zou, H., et al. 2023. Nat Cell Biol. 25: 493-507. PMID: 36849558
5. Heterozygous Dab1 Null Mutation Disrupts Neocortical and Hippocampal Development.  |  Honda, T., et al. 2023. eNeuro. 10: PMID: 36941061
6. ApoER2-Dab1 disruption as the origin of pTau-associated neurodegeneration in sporadic Alzheimer's disease.  |  Ramsden, CE., et al. 2023. Acta Neuropathol Commun. 11: 197. PMID: 38093390
7. The heterotrimeric kinesin-2 family member KIF3A directly binds to disabled-1 (Dab1).  |  Kim, MH., et al. 2024. BMB Rep. 57: 447-452. PMID: 38919020
8. The reeler gene-associated antigen on Cajal-Retzius neurons is a crucial molecule for laminar organization of cortical neurons.  |  Ogawa, M., et al. 1995. Neuron. 14: 899-912. PMID: 7748558
9. Mouse disabled (mDab1): a Src binding protein implicated in neuronal development.  |  Howell, BW., et al. 1997. EMBO J. 16: 121-32. PMID: 9009273
10. Neuronal position in the developing brain is regulated by mouse disabled-1.  |  Howell, BW., et al. 1997. Nature. 389: 733-7. PMID: 9338785
11. Disabled-2 (Dab2) is an SH3 domain-binding partner of Grb2.  |  Xu, XX., et al. 1998. Oncogene. 16: 1561-9. PMID: 9569023
12. Disabled-1 acts downstream of Reelin in a signaling pathway that controls laminar organization in the mammalian brain.  |  Rice, DS., et al. 1998. Development. 125: 3719-29. PMID: 9716537

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dab1 Antibody (G-5) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Dab1 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dab1 Antibody (G-5) Specifications

Alternate Product Name: Dab1

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 451-553

Epitope Target: Dab1

Epitope Species: human

Product Reactivity/Detection: Dab1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Dab1 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin