Cytokeratin 7 Antibody (5F282) recommended for detection of Cytokeratin 7 of mouse, rat, human, hamster, canine and porcine origin by WB, IP, IF, IHC(P) and FCM

Cytokeratin 7 Antibody (5F282): sc-70936

Cytokeratin 7 Antibody (5F282) is rated 4.0 out of 5 by 3.
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Datasheets

    • Cytokeratin 7 Antibody (5F282) is a mouse monoclonal IgG1 κ, cited in 9 publications, provided at 200 µg/ml
    • raised against T24 cells
    • recommended for detection of Cytokeratin 7 of mouse, rat, human, hamster, canine and porcine origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cytokeratin 7 (5F282): sc-70936.

Can Cytokeratin 7 (5F282): sc-70936 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Cytokeratin 7 (5F282): sc-70936 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from great monoclonal for WB I used this antibody for WB in ovarian cancer cell. Good signal at the right size. (used at 1/300 for a better signal)
Date published: 2018-11-22
Rated 4 out of 5 by from IF: Very good staining in HeLa cells IF: Very good staining in HeLa cells. -SCBT QC
Date published: 2015-04-13
Rated 4 out of 5 by from Works well for Western in HeLa and T24 whole Works well for Western in HeLa and T24 whole cell lysates. -SCBT QC
Date published: 2015-03-30
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