Cytokeratin 7 Antibody (RCK105): sc-23876

Cytokeratin 7 Antibody (RCK105) is a mouse monoclonal IgG1 kappa light chain antibody designed to target Cytokeratin 7, also known as KRT7 or sarcolectin. Anti-Cytokeratin 7 antibody (RCK105) is raised against T24 cells and demonstrates broad species reactivity, including mouse, rat, human, hamster, canine, and porcine, making it suitable for a variety of applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Cytokeratin 7 is an intermediate filament protein located within the cytoskeleton of epithelial cells, where Cytokeratin 7 plays a critical role in maintaining cellular structural integrity and facilitating tissue differentiation and specialization. The precise localization of Cytokeratin 7 within the cell is essential for the stability and resilience of epithelial tissues, and Cytokeratin 7 serves as a valuable marker for identifying and characterizing both normal and malignant epithelial cells. Anti-Cytokeratin 7 antibody (RCK105) enables researchers to effectively study epithelial cell biology, monitor differentiation processes, and investigate the molecular mechanisms underlying various cancers. Cytokeratin 7 (RCK105) monoclonal antibody is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and a range of Alexa Fluor® conjugates, providing flexibility to meet diverse experimental needs. Additionally, Cytokeratin 7 plays a significant role in the structural framework of epithelial cells, aiding in the diagnosis and classification of tumors by highlighting specific cellular structures.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cytokeratin 7 Antibody (RCK105) References:

1. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas.  |  Ramaekers, F., et al. 1990. Am J Pathol. 136: 641-55. PMID: 1690512
2. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.  |  Vojtĕsek, B., et al. 1990. Neoplasma. 37: 333-42. PMID: 1695327
3. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.  |  Bártek, J., et al. 1991. J Pathol. 164: 215-24. PMID: 1716305
4. Expression of simple epithelial keratins 8 and 18 in epidermal neoplasia.  |  Markey, AC., et al. 1991. J Invest Dermatol. 97: 763-70. PMID: 1717607
5. Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues.  |  van Niekerk, CC., et al. 1991. J Pathol. 165: 145-52. PMID: 1720817
6. Keratin antigens in differentiating skin.  |  Lane, EB., et al. 1985. Ann N Y Acad Sci. 455: 241-58. PMID: 2417515
7. Differential diagnosis of gastrointestinal carcinomas by using monoclonal antibodies specific for individual keratin polypeptides.  |  Osborn, M., et al. 1986. Lab Invest. 55: 497-504. PMID: 2429069
8. Fusobacterium nucleatum promotes colorectal cancer metastasis by modulating KRT7-AS/KRT7.  |  Chen, S., et al. 2020. Gut Microbes. 11: 511-525. PMID: 31910722
9. Cytokeratin 7 and cytokeratin 20 expression in cancer: A tissue microarray study on 15,424 cancers.  |  Dum, D., et al. 2022. Exp Mol Pathol. 126: 104762. PMID: 35390310
10. Evaluation of Cytokeratin-7 and Cytokeratin-19 Expression Relationship with Gleason Score in Prostatic Adenocarcinoma.  |  Soltanipur, M., et al. 2023. Iran J Pathol. 18: 363-369. PMID: 37942200
11. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.  |  Moll, R., et al. 1982. Cell. 31: 11-24. PMID: 6186379
12. Keratins and the skin.  |  Fuchs, E. 1995. Annu Rev Cell Dev Biol. 11: 123-53. PMID: 8689554

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 7 Antibody (RCK105) has 52 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 7 Antibody (RCK105) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 7 Antibody (RCK105) Specifications

Alternate Product Name: KRT7

Product Clone Name: RCK105

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Cytokeratin 7

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 7

Species Reactivity/Detection: mouse, rat, human, hamster, canine, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 52 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 7 Antibody (RCK105): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin