Cytokeratin 5/8 Antibody (5F295): sc-70928
- Cytokeratin 5/8 Antibody (5F295) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against the human lung cancer cell line, MR21
- recommended for detection of Cytokeratin 5/8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; may cross-react with Cytokeratin 18
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See pan-Cytokeratin (C11): sc-8018 for Cytokeratin 5/8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Cytokeratin 5/8 Antibody (5F295) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 5/8 Antibody (5F295) (see ordering information below).
QUICK LINKS
SEE ALSO...
Cytokeratin 5/8 Antibody (5F295) is a mouse monoclonal IgG1 antibody that detects Cytokeratin 5 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Cytokeratin 5 is a vital component of the cytoskeletal structure in epithelial cells, primarily found in the basal layer of stratified squamous epithelium, where Cytokeratin 5 plays a crucial role in maintaining cellular integrity and resilience against mechanical stress. Cytokeratin 5′s location is significant as anti-Cytokeratin 5 antibody (5F295) contributes to the formation of a robust barrier that protects underlying tissues from injury and infection. Additionally, Cytokeratin 5 is essential for the differentiation of epithelial cells, and Cytokeratin 5 expression is often used as a marker for identifying basal cell carcinomas and other malignancies. Mutations in the gene encoding Cytokeratin 5 (KRT5) can lead to severe skin disorders such as epidermolysis bullosa, highlighting Cytokeratin 5′s importance in both normal physiology and disease states. Anti-Cytokeratin 5 antibody (5F295) is therefore an invaluable tool for researchers studying epithelial biology and related pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cytokeratin 5/8 Antibody (5F295) References:
- Cytokeratin 5/6 immunostaining in cutaneous adnexal neoplasms and metastatic adenocarcinoma. | Plumb, SJ., et al. 2004. Am J Dermatopathol. 26: 447-51. PMID: 15618924
- Cloning and sequence of cDNA for human placental cytokeratin 8. Regulation of the mRNA in trophoblastic cells by cAMP. | Yamamoto, R., et al. 1990. Mol Endocrinol. 4: 370-4. PMID: 1692965
- Tumor suppressor SMAR1 downregulates Cytokeratin 8 expression by displacing p53 from its cognate site. | Pavithra, L., et al. 2009. Int J Biochem Cell Biol. 41: 862-71. PMID: 18822384
- Cytokeratin 8 is expressed in human corneoconjunctival epithelium, particularly in limbal epithelial cells. | Merjava, S., et al. 2011. Invest Ophthalmol Vis Sci. 52: 787-94. PMID: 20926822
- Cytokeratin 5/6 expression in benign and malignant breast lesions. | Bhalla, A., et al. 2010. Indian J Pathol Microbiol. 53: 676-80. PMID: 21045391
- Utility of cytokeratin 5/6, cytokeratin 20, and p16 in the diagnosis of reactive urothelial atypia and noninvasive component of urothelial neoplasia. | Edgecombe, A., et al. 2012. Appl Immunohistochem Mol Morphol. 20: 264-71. PMID: 22498670
- Expression of c-kit and Cytokeratin 5 in the Submandibular Gland after Release of Long-Term Ligation of the Main Excretory Duct in Mice. | Watanabe, H., et al. 2017. Acta Histochem Cytochem. 50: 111-118. PMID: 28744028
- β cells can be generated from cytokeratin 5-positive cells after cerulein-induced pancreatitis in adult mice. | Shi, Q., et al. 2018. Biochem Biophys Res Commun. 496: 114-119. PMID: 29307825
- Cytokeratin 5/6 expression in pT1 bladder cancer predicts intravesical recurrence in patients treated with bacillus Calmette-Guérin instillation. | Yamamoto, Y., et al. 2022. Pathology. 54: 700-706. PMID: 35527047
- Cytokeratin 8, 18 and 19 in endometrial epithelium of Norplant and norethisterone enanthate injectable progestogen contraceptive users. | Wonodirekso, S., et al. 1996. Hum Reprod. 11 Suppl 2: 144-9. PMID: 8982756
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 5/8 Antibody (5F295) | sc-70928 | 200 µg/ml | $322.00 | |||
Cytokeratin 5/8 Antibody (5F295): m-IgG Fc BP-HRP Bundle | sc-539323 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cytokeratin 5/8 Antibody (5F295) FITC | sc-70928 FITC | 200 µg/ml | $336.00 | |||
Cytokeratin 5/8 Antibody (5F295) PE | sc-70928 PE | 200 µg/ml | $349.00 |