Cytokeratin 5 Antibody (RCK103): sc-32721

Cytokeratin 5 Antibody (RCK103) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cytokeratin 5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Cytokeratin 5 antibody (RCK103) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratin 5 is a vital component of the cytoskeleton in epithelial cells, primarily located in the basal layer of stratified epithelia, where Cytokeratin 5 plays a crucial role in providing mechanical support and resilience to cellular stress. This structural positioning is essential for maintaining the integrity of epithelial tissues, particularly in areas subject to friction and abrasion, such as the skin and respiratory tract. Cytokeratin 5 expression can serve as a marker for certain types of epithelial tumors, aiding in the diagnosis and characterization of malignancies. Additionally, mutations in the cytokeratin 5 gene (KRT5) are linked to the autosomal dominant disorder epidermolysis bullosa, highlighting Cytokeratin 5′s importance in both normal physiology and disease states.

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Cytokeratin 5 Antibody (RCK103) References:

1. Cytokeratin 5-Positive Cells Represent a Therapy Resistant subpopulation in Epithelial Ovarian Cancer.  |  Corr, BR., et al. 2015. Int J Gynecol Cancer. 25: 1565-73. PMID: 26495758
2. Cytokeratin 5 determines maturation of the mammary myoepithelium.  |  Deckwirth, V., et al. 2021. iScience. 24: 102413. PMID: 34007958
3. Cytokeratin 5 and cytokeratin 20 inversely correlate with tumour grading in Ta non-muscle-invasive bladder cancer.  |  Muilwijk, T., et al. 2021. J Cell Mol Med. 25: 7890-7900. PMID: 34184816
4. Cytokeratin 5 and cytokeratin 6 expressions are unconnected in normal and cancerous tissues and have separate diagnostic implications.  |  Völkel, C., et al. 2022. Virchows Arch. 480: 433-447. PMID: 34559291
5. Clinicopathological features of cytokeratin 5-positive pulmonary adenocarcinoma.  |  Terada, K., et al. 2023. Histopathology. 82: 439-453. PMID: 36239561
6. Evaluation of a new tumor marker for cytokeratin 8 and 18 fragments in healthy individuals and prostate cancer patients.  |  Silén, A., et al. 1994. Prostate. 24: 326-32. PMID: 7516071
7. Epidermolysis bullosa simplex: a keratin 5 mutation is a fully dominant allele in epidermal cytoskeleton function.  |  Stephens, K., et al. 1995. Am J Hum Genet. 56: 577-85. PMID: 7534039
8. A novel IRMA and ELISA for quantifying cytokeratin 8 and 18 fragments in the sera of healthy individuals and cancer patients.  |  Silén, A., et al. 1995. Scand J Clin Lab Invest. 55: 153-61. PMID: 7545308
9. Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview.  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
10. Serum and tissue distribution of a fragment of cytokeratin 19 (cyfra 21-1) in lung cancer patients.  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
11. Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis.  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
12. Cytokeratin expression, fibrillar organization, and subtle function in liver cells.  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 5 Antibody (RCK103) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 5 Antibody (RCK103) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 5 Antibody (RCK103) Specifications

Alternate Product Name: Keratin 5

Product Clone Name: RCK103

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Cytokeratin 5

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 5 Antibody (RCK103): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin