Cytokeratin 4 Antibody (5F132) recommended for detection of Cytokeratin 4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Cytokeratin 4 Antibody (5F132): sc-70919

Cytokeratin 4 Antibody (5F132) is rated 3.0 out of 5 by 1.
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Datasheets

    • Cytokeratin 4 Antibody (5F132) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against cytokeratin preparation from esophagus cells of human origin
    • recommended for detection of Cytokeratin 4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and porcine, feline and canine
    • See pan-Cytokeratin (C11): sc-8018 for Cytokeratin 4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can sc-70919: Cytokeratin 4 (5F132) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, sc-70919: Cytokeratin 4 (5F132) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Positive WB data in HeLa samples Positive WB data in HeLa samples.
Date published: 2015-03-03
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