Cytokeratin 17 Antibody (E-4): sc-393002

Cytokeratin 17 Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Cytokeratin 17 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Cytokeratin 17 antibody (E-4) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Cytokeratin 17, also known as KRT17, is a crucial member of the cytokeratin subfamily of intermediate filament proteins, comprising 432 amino acids. KRT17 is predominantly expressed in the basal cells of complex epithelia, including the nail bed, hair follicle, and sebaceous glands, but is absent in stratified or simple epithelia. KRT17′s unique expression pattern plays a vital role in maintaining the structural integrity and function of epithelial tissues. KRT17 interacts with various proteins, including the adaptor protein 14-3-3-sigma, which is essential for regulating cell growth and size by mediating protein synthesis. Mutations in the KRT17 gene can lead to impaired protein translation and reduced keratinocyte size, which is associated with decreased Akt/mTOR signaling activity. Cytokeratin 17 monoclonal antibody (E-4) may serve as a valuable marker for identifying cervical stem cells, as well as in the study of squamous cell carcinoma of the larynx, respiratory syncytial virus, and transitional cell carcinomas of the human urinary tract.

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Cytokeratin 17 Antibody (E-4) References:

1. Cytokeratin 17 is expressed in cells infected with respiratory syncytial virus via NF-kappaB activation and is associated with the formation of cytopathic syncytia.  |  Domachowske, JB., et al. 2000. J Infect Dis. 182: 1022-8. PMID: 10979895
2. Dithranol and dimethylfumarate suppress the interferon-gamma-induced up-regulation of cytokeratin 17 as a putative psoriasis autoantigen in vitro.  |  Bonnekoh, B., et al. 2001. Skin Pharmacol Appl Skin Physiol. 14: 217-25. PMID: 11464104
3. Phosphorylation of cytokeratin 17 by herpes simplex virus type 2 US3 protein kinase.  |  Murata, T., et al. 2002. Microbiol Immunol. 46: 707-19. PMID: 12477250
4. Cytokeratin 17 and p63 are markers of the HPV target cell, the cervical stem cell.  |  Martens, JE., et al. 2004. Anticancer Res. 24: 771-5. PMID: 15161025
5. Cytokeratin-17 as a potential marker for squamous cell carcinoma of the larynx.  |  Cohen-Kerem, R., et al. 2004. Ann Otol Rhinol Laryngol. 113: 821-7. PMID: 15535145
6. Association of cytokeratin 17 expression with differentiation in oral squamous cell carcinoma.  |  Kitamura, R., et al. 2012. J Cancer Res Clin Oncol. 138: 1299-310. PMID: 22466643
7. BerEp4, cytokeratin 14, and cytokeratin 17 immunohistochemical staining aid in differentiation of basaloid squamous cell carcinoma from basal cell carcinoma with squamous metaplasia.  |  Linskey, KR., et al. 2013. Arch Pathol Lab Med. 137: 1591-8. PMID: 24168496
8. Decreasing cytokeratin 17 expression in head and neck cancer predicts nodal metastasis and poor prognosis: The first evidence.  |  Xu, ES., et al. 2018. Clin Otolaryngol. 43: 1010-1018. PMID: 29495101
9. Activation of the silent human cytokeratin 17 pseudogene-promoter region by cryptic enhancer elements of the cytokeratin 17 gene.  |  Troyanovsky, SM. and Leube, RE. 1994. Eur J Biochem. 225: 61-9. PMID: 7523124
10. Immunohistochemical localization of cytokeratin 17 in transitional cell carcinomas of the human urinary tract.  |  Guelstein, VI., et al. 1993. Virchows Arch B Cell Pathol Incl Mol Pathol. 64: 1-5. PMID: 7691361
11. Control of cytokeratin 17 expression by interferon gamma.  |  Vogel, U. and Böttger, EC. 1995. Immunobiology. 193: 322-7. PMID: 8530161

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 17 Antibody (E-4) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 17 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 17 Antibody (E-4) Specifications

Alternate Product Name: KRT17

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 392-432

Epitope Target: Cytokeratin 17

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 17

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 17 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin