CYP4A11 Antibody (M25-P2A10): sc-101385
- CYP4A11 Antibody (M25-P2A10) is a mouse monoclonal IgG1 κ CYP4A11 antibody provided at 200 µg/ml
- raised against a synthetic peptide corresponding to amino acids 499-507 of CYP4A11 of human origin
- CYP4A11 Antibody (M25-P2A10) is recommended for detection of CYP4A11 of human origin by WB, IP and ELISA; may cross-react with CYP4A22
- Anti-CYP4A11 Antibody (M25-P2A10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CYP4A11 Antibody (M25-P2A10) for WB applications. These reagents are now offered in bundles with CYP4A11 Antibody (M25-P2A10) (see ordering information below).
CYP4A11 Antibody (M25-P2A10) is a mouse monoclonal IgG1 kappa light chain antibody that detects CYP4A11 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CYP4A11 antibody (M25-P2A10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP4A11, also known as CP4Y, CYP4A2, P450HL-omega, or CYP4AII, is a 519 amino acid enzyme primarily expressed in the kidney and liver, where CYP4A11 is localized to the membrane of the endoplasmic reticulum. CYP4A11 plays a crucial role in fatty acid metabolism by catalyzing the oxygen-dependent hydroxylation of medium-chain fatty acids, such as myristate, laurate, and palmitate, utilizing heme as a cofactor. CYP4A11 metabolizes arachidonic acid into hydroxyeicosatetraenoic acid and epoxyeicosatrienoic acid, which are significant regulators of blood pressure and mediators of various signaling pathways. Due to alternative splicing, CYP4A11 exists as two isoforms, which may contribute to functional diversity and regulatory mechanisms in lipid metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYP4A11 Antibody (M25-P2A10) References:
- Homology modeling and substrate binding study of human CYP4A11 enzyme. | Chang, YT. and Loew, GH. 1999. Proteins. 34: 403-15. PMID: 10024026
- Formation of 20-hydroxyeicosatetraenoic acid, a vasoactive and natriuretic eicosanoid, in human kidney. Role of Cyp4F2 and Cyp4A11. | Lasker, JM., et al. 2000. J Biol Chem. 275: 4118-26. PMID: 10660572
- Covalently linked heme in cytochrome p4504a fatty acid hydroxylases. | Hoch, U. and Ortiz De Montellano, PR. 2001. J Biol Chem. 276: 11339-46. PMID: 11139583
- Functional variant of CYP4A11 20-hydroxyeicosatetraenoic acid synthase is associated with essential hypertension. | Gainer, JV., et al. 2005. Circulation. 111: 63-9. PMID: 15611369
- A single nucleotide polymorphism in the CYP4F2 but not CYP4A11 gene is associated with increased 20-HETE excretion and blood pressure. | Ward, NC., et al. 2008. Hypertension. 51: 1393-8. PMID: 18391101
- Haplotype-based case study of human CYP4A11 gene and cerebral infarction in Japanese subject. | Fu, Z., et al. 2008. Endocrine. 33: 215-22. PMID: 18484194
- The V433M variant of the CYP4F2 is associated with ischemic stroke in male Swedes beyond its effect on blood pressure. | Fava, C., et al. 2008. Hypertension. 52: 373-80. PMID: 18574070
- Complete cDNA sequence and cDNA-directed expression of CYP4A11, a fatty acid omega-hydroxylase expressed in human kidney. | Imaoka, S., et al. 1993. DNA Cell Biol. 12: 893-9. PMID: 8274222
- Metabolism of arachidonic acid to 20-hydroxy-5,8,11, 14-eicosatetraenoic acid by P450 enzymes in human liver: involvement of CYP4F2 and CYP4A11. | Powell, PK., et al. 1998. J Pharmacol Exp Ther. 285: 1327-36. PMID: 9618440
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP4A11 Antibody (M25-P2A10) | sc-101385 | 200 µg/ml | $322.00 | |||
CYP4A11 Antibody (M25-P2A10): m-IgG Fc BP-HRP Bundle | sc-528717 | 200 µg Ab; 10 µg BP | $361.00 | |||
CYP4A11 Antibody (M25-P2A10): m-IgGκ BP-HRP Bundle | sc-521188 | 200 µg Ab, 40 µg BP | $361.00 | |||
CYP4A11 Antibody (M25-P2A10): m-IgG1 BP-HRP Bundle | sc-543087 | 200 µg Ab; 20 µg BP | $361.00 | |||
CYP4A11 Antibody (M25-P2A10) AC | sc-101385 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CYP4A11 Antibody (M25-P2A10) HRP | sc-101385 HRP | 200 µg/ml | $322.00 | |||
CYP4A11 Antibody (M25-P2A10) FITC | sc-101385 FITC | 200 µg/ml | $336.00 | |||
CYP4A11 Antibody (M25-P2A10) PE | sc-101385 PE | 200 µg/ml | $349.00 | |||
CYP4A11 Antibody (M25-P2A10) Alexa Fluor® 488 | sc-101385 AF488 | 200 µg/ml | $364.00 | |||
CYP4A11 Antibody (M25-P2A10) Alexa Fluor® 546 | sc-101385 AF546 | 200 µg/ml | $364.00 | |||
CYP4A11 Antibody (M25-P2A10) Alexa Fluor® 594 | sc-101385 AF594 | 200 µg/ml | $364.00 | |||
CYP4A11 Antibody (M25-P2A10) Alexa Fluor® 647 | sc-101385 AF647 | 200 µg/ml | $364.00 | |||
CYP4A11 Antibody (M25-P2A10) Alexa Fluor® 680 | sc-101385 AF680 | 200 µg/ml | $364.00 | |||
CYP4A11 Antibody (M25-P2A10) Alexa Fluor® 790 | sc-101385 AF790 | 200 µg/ml | $364.00 |