Cyclophilin 40 Antibody (C-11) recommended for detection of Cyclophilin 40 of mouse, rat and human origin by WB, IP, IF and ELISA

Cyclophilin 40 Antibody (C-11): sc-137216

Cyclophilin 40 Antibody (C-11) is rated 4.5 out of 5 by 6.
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Datasheets

    • Anti-Cyclophilin 40 Antibody (C-11) is a mouse monoclonal IgG2b (kappa light chain) Cyclophilin 40 antibody provided at 200 µg/ml
    • raised against amino acids 186-370 mapping at the C-terminus of Cyclophilin 40 of human origin
    • Anti-Cyclophilin 40 Antibody (C-11) is recommended for detection of Cyclophilin 40 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Cyclophilin 40 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cyclophilin 40 (C-11): sc-137216.

What is the recommended fixation method for immunofluorescence staining with Cyclophilin 40 (C-11): sc-137216 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Lab Manager I got this a month ago as a sample, It works great on WB, only shows one clean band, no background.
Date published: 2019-09-25
Rated 3 out of 5 by from Not quite strong band 1:500 diluted for wb of rat heart protein ,the band is not quite strong,I will try 1:200 diluted again.
Date published: 2017-09-27
Rated 5 out of 5 by from specific staining. This antibody gave a positive staining when use it detect Cyclophilin 40 in mouse lung tissue, I obtained specific staining.
Date published: 2017-05-13
Rated 4 out of 5 by from This has worked well in WB This has worked well in WB.
Date published: 2015-10-27
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmic Produced superb immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-02-14
Rated 5 out of 5 by from Good Western Blot data of Cyclophilin 40 Good Western Blot data of Cyclophilin 40 expression in non-transfected 293T, mouse Cyclophilin 40 transfected 293T, JAR and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2013-05-12
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