Anti-Cyclophilin 40 Antibody (C-11) is a mouse monoclonal IgG2b (kappa light chain) Cyclophilin 40 antibody provided at 200 µg/ml
raised against amino acids 186-370 mapping at the C-terminus of Cyclophilin 40 of human origin
Anti-Cyclophilin 40 Antibody (C-11) is recommended for detection of Cyclophilin 40 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-Cyclophilin 40 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cyclophilin 40 (C-11): sc-137216.
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Cyclophilin 40 Antibody (C-11) is a high quality monoclonal Cyclophilin 40 antibody (also designated Cyclophilin 40 antibody) suitable for the detection of the Cyclophilin 40 protein of mouse, rat and human origin. Cyclophilin 40 Antibody (C-11) is available as both the non-conjugated anti-Cyclophilin 40 antibody form, as well as multiple conjugated forms of anti-Cyclophilin 40 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of cyclosporin A (CsA) and is encoded by a single unique gene conserved from yeast to humans. Cyclophilin B (CyPB) is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. Cyclophilin 40 (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It is a widely expressed cytoplasmic protein.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with Cyclophilin 40 (C-11): sc-137216 monoclonal antibody?
Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
Rated 5 out of
Lab ManagerI got this a month ago as a sample, It works great on WB, only shows one clean band, no background.
Date published: 2019-09-25
Rated 3 out of
Not quite strong band1:500 diluted for wb of rat heart protein ,the band is not quite strong,I will try 1:200 diluted again.
Date published: 2017-09-27
Rated 5 out of
specific staining.This antibody gave a positive staining when use it detect Cyclophilin 40 in mouse lung tissue, I obtained specific staining.
Date published: 2017-05-13
Rated 4 out of
This has worked well in WBThis has worked well in WB.
Date published: 2015-10-27
Rated 5 out of
Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-02-14
Rated 5 out of
Good Western Blot data of Cyclophilin 40Good Western Blot data of Cyclophilin 40 expression in non-transfected 293T, mouse Cyclophilin 40 transfected 293T, JAR and NIH/3T3 whole cell lysates. -SCBT QC
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