cyclin F Antibody (B-6): sc-515207
- cyclin F Antibody (B-6) is a mouse monoclonal IgG2b κ cyclin F antibody, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 774-798 at the C-terminus of cyclin F of human origin
- cyclin F Antibody (B-6) is recommended for detection of cyclin F of human origin by WB, IP, IF and ELISA
- Anti-cyclin F Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin F Antibody (B-6) for WB applications. These reagents are now offered in bundles with cyclin F Antibody (B-6) (see ordering information below).
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Cyclin F Antibody (B-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects cyclin F of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cyclin F antibody (B-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cyclin F plays a crucial role in cell cycle regulation, being the largest member of the cyclin family, and contains a unique PEST-rich C-terminus along with a cyclin box region closely related to cyclins A and B. Cyclin F′s function is particularly important in controlling the G2 to M transition, a critical phase in cell division, where proper regulation ensures accurate DNA replication and distribution to daughter cells. Cyclin F expression occurs throughout the cell cycle but shows dynamic fluctuations, peaking during the G2 phase, which is essential for preparing cells for mitosis. Cyclin F′s regulated subcellular localization, primarily in the nucleus with notable perinuclear staining in some cells, underscores its significance in cellular processes and highlights its potential as a target for studying cell cycle-related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin F Antibody (B-6) References:
- D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA. | Xiong, Y., et al. 1992. Cell. 71: 505-14. PMID: 1358458
- Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins. | Matsushime, H., et al. 1992. Cell. 71: 323-34. PMID: 1423597
- Cyclin F disruption compromises placental development and affects normal cell cycle execution. | Tetzlaff, MT., et al. 2004. Mol Cell Biol. 24: 2487-98. PMID: 14993286
- Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes. | Fang, F. and Newport, JW. 1991. Cell. 66: 731-42. PMID: 1652371
- Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. | Koff, A., et al. 1991. Cell. 66: 1217-28. PMID: 1833068
- Cyclin A is required for the onset of DNA replication in mammalian fibroblasts. | Girard, F., et al. 1991. Cell. 67: 1169-79. PMID: 1836977
- Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. | Pines, J. and Hunter, T. 1990. Nature. 346: 760-3. PMID: 2143810
- Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control. | Klein, DK., et al. 2015. Nat Commun. 6: 5800. PMID: 25557911
- Cyclin F is involved in response to cisplatin treatment in melanoma cell lines. | Krajewski, A., et al. 2020. Oncol Rep. 43: 765-772. PMID: 32020229
- ALS/FTD-associated mutation in cyclin F inhibits ER-Golgi trafficking, inducing ER stress, ERAD and Golgi fragmentation. | Ragagnin, AMG., et al. 2023. Sci Rep. 13: 20467. PMID: 37993492
- Human cyclin F. | Bai, C., et al. 1994. EMBO J. 13: 6087-98. PMID: 7813445
- Cyclin G: a new mammalian cyclin with homology to fission yeast Cig1. | Tamura, K., et al. 1993. Oncogene. 8: 2113-8. PMID: 8336937
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin F Antibody (B-6) | sc-515207 | 200 µg/ml | $316.00 | |||
cyclin F Antibody (B-6): m-IgGκ BP-HRP Bundle | sc-524971 | 200 µg Ab, 40 µg BP | $354.00 | |||
cyclin F Antibody (B-6): m-IgG2b BP-HRP Bundle | sc-549202 | 200 µg Ab; 10 µg BP | $354.00 | |||
cyclin F Antibody (B-6) AC | sc-515207 AC | 500 µg/ml, 25% agarose | $416.00 | |||
cyclin F Antibody (B-6) HRP | sc-515207 HRP | 200 µg/ml | $316.00 | |||
cyclin F Antibody (B-6) FITC | sc-515207 FITC | 200 µg/ml | $330.00 | |||
cyclin F Antibody (B-6) PE | sc-515207 PE | 200 µg/ml | $343.00 | |||
cyclin F Antibody (B-6) Alexa Fluor® 488 | sc-515207 AF488 | 200 µg/ml | $357.00 | |||
cyclin F Antibody (B-6) Alexa Fluor® 546 | sc-515207 AF546 | 200 µg/ml | $357.00 | |||
cyclin F Antibody (B-6) Alexa Fluor® 594 | sc-515207 AF594 | 200 µg/ml | $357.00 | |||
cyclin F Antibody (B-6) Alexa Fluor® 647 | sc-515207 AF647 | 200 µg/ml | $357.00 | |||
cyclin F Antibody (B-6) Alexa Fluor® 680 | sc-515207 AF680 | 200 µg/ml | $357.00 | |||
cyclin F Antibody (B-6) Alexa Fluor® 790 | sc-515207 AF790 | 200 µg/ml | $357.00 | |||
cyclin F (B-6) Neutralizing Peptide | sc-515207 P | 100 µg/0.5 ml | $68.00 |