cyclin D1 Antibody (A-12): sc-8396

Cyclin D1 Antibody (A-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects cyclin D1 of mouse, rat, and human origin through various applications including western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay. Cyclin D1 (A-12) antibody is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. Cyclin D1 plays a pivotal role in cell cycle regulation, particularly during the G1 phase, where cyclin D1 is essential for the transition from G1 to S phase. This transition is crucial for cellular proliferation, as cells prepare for DNA synthesis. Dysregulation of cyclin D1 is associated with various cancers, leading to uncontrolled cell division. Cyclin D1 binds and activates cyclin-dependent kinases (CDKs), facilitating phosphorylation of the retinoblastoma protein, which releases E2F transcription factors that drive expression of genes necessary for DNA replication. Understanding cyclin D1 function is vital for elucidating mechanisms of tumorigenesis and developing targeted cancer therapies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

cyclin D1 Antibody (A-12) References:

1. ras activity and cyclin D1 expression: an essential mechanism of mouse skin tumor development.  |  Rodriguez-Puebla, ML., et al. 1999. Mol Carcinog. 24: 1-6. PMID: 10029404
2. Cyclin D1 in breast premalignancy and early breast cancer: implications for prevention and treatment.  |  Zhou, Q., et al. 2001. Cancer Lett. 162: 3-17. PMID: 11121857
3. Cyclin D1: mechanism and consequence of androgen receptor co-repressor activity.  |  Petre, CE., et al. 2002. J Biol Chem. 277: 2207-15. PMID: 11714699
4. Cyclin A in cell cycle control and cancer.  |  Yam, CH., et al. 2002. Cell Mol Life Sci. 59: 1317-26. PMID: 12363035
5. Cyclin D1 repression of peroxisome proliferator-activated receptor gamma expression and transactivation.  |  Wang, C., et al. 2003. Mol Cell Biol. 23: 6159-73. PMID: 12917338
6. Cyclin D1 gene (CCND1) mutations in endometrial cancer.  |  Moreno-Bueno, G., et al. 2003. Oncogene. 22: 6115-8. PMID: 12955092
7. Cyclin D1 induction of cellular migration requires p27(KIP1).  |  Li, Z., et al. 2006. Cancer Res. 66: 9986-94. PMID: 17047061
8. Cyclin D1 regulates hepatic estrogen and androgen metabolism.  |  Mullany, LK., et al. 2010. Am J Physiol Gastrointest Liver Physiol. 298: G884-95. PMID: 20338923
9. Cyclin D1 inactivation extends proliferation and alters histogenesis in the postnatal mouse retina.  |  Das, G., et al. 2012. Dev Dyn. 241: 941-52. PMID: 22434780
10. Cyclin D1 overexpression in proliferation centres of small lymphocytic lymphoma/chronic lymphocytic leukaemia.  |  Teixeira Mendes, LS., et al. 2017. J Clin Pathol. 70: 899-902. PMID: 28408436
11. Identifying Cyclin A/Cdk1 Substrates in Mitosis in Human Cells.  |  Dumitru, AMG. and Compton, DA. 2022. Methods Mol Biol. 2415: 175-182. PMID: 34972954
12. Mechanism of cyclin D1 (CCND1, PRAD1) overexpression in human cancer cells: analysis of allele-specific expression.  |  Hosokawa, Y. and Arnold, A. 1998. Genes Chromosomes Cancer. 22: 66-71. PMID: 9591636

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cyclin D1 Antibody (A-12) has 732 citations in a variety of scientific publications.

Primary antibodies like Anti-cyclin D1 Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cyclin D1 Antibody (A-12) Specifications

Alternate Product Name: CCND1

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-295

Epitope Target: cyclin D1

Epitope Species: human

Product Reactivity/Detection: cyclin D1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 732 citations in various scientific publications

Available Conjugates/Forms for cyclin D1 Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine

The preferred cyclin D1 antibody is cyclin D1 Antibody (DCS-6): sc-20044.