Anti-cyclin D1 Antibody (A-12) recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-cyclin D1 Antibody (A-12): sc-8396

Anti-cyclin D1 Antibody (A-12) is rated 4.3 out of 5 by 68.
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Datasheets

    • Anti-cyclin D1 Antibody (A-12) is a mouse monoclonal IgG2b κ cyclin D1 antibody, cited in 591 publications, provided at 200 µg/ml
    • raised against amino acids 1-295 representing full length cyclin D1 of human origin
    • Anti-cyclin D1 Antibody (A-12) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; may cross-react with cyclin D2; non cross-reactive with cyclin D3
    • Anti-cyclin D1 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin D1 (A-12): sc-8396.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for cyclin D1 Antibody (A-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin D1 Antibody (A-12) (see ordering information below).
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I am looking for a CDK1 antibody suitable to detect a C-terminal GFP CDK1 fusion protein on WB, where CDK1 is truncated resulting in a loss of the last 5 amino acids of CDK1. Which antibody would you recommend? Thanks!

Asked by: Sebastian Scheich
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-02-04

What is the recommended secondary detection reagent for cyclin D1 Antibody (A-12) in Western blot applications?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-29
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Rated 4 out of 5 by from Ciclin D1 antibody Works excellent in western blot for human Cyclin D1 detection
Date published: 2021-06-02
Rated 4 out of 5 by from Works for WB It works good for detecting rat liver protein by western blot
Date published: 2021-01-18
Rated 5 out of 5 by from 功能很出色 之前用过别的家的抗体不行,换了这家的cyclin D1,结果一下子就出来了.很给力,希望更多人受益。
Date published: 2021-01-05
Rated 3 out of 5 by from Decent Western detection in rat INS-1 cells 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong band of ~38 kD and several non-specific bands. Recommend using sc-20044 instead. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 2 out of 5 by from Not really work for IF I used 1:20 dilution for IF staining of chondrocytes on cryo sections, but it does not really work. The one that I used is conjugated to Alexa 647.
Date published: 2019-05-06
Rated 4 out of 5 by from Working for WB It works for dettecting mouse and human protein by western blot
Date published: 2019-01-17
Rated 5 out of 5 by from UPENN Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 5 out of 5 by from Worked Great!! Worked great for human and mouse tissue samples when probing at 1:200 via WB. Highly recommend!
Date published: 2018-03-22
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