CCNB1/cyclin B1 Antibody (GNS1): sc-245

Cyclin B1 Antibody (GNS1) is a mouse monoclonal IgG1 kappa light chain antibody raised against a recombinant protein corresponding to human cyclin B1, also known as CCNB1. Cyclin B1 monoclonal antibody (GNS1) reacts with mouse, rat, and human species and is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry on paraffin-embedded sections (IHCP). Cyclin B1 is a regulatory protein essential for controlling the cell cycle at the G2/M transition, forming a complex with the cyclin-dependent kinase CDK1 to create the maturation-promoting factor (MPF). This complex initiates mitosis in eukaryotic cells, making cyclin B1 crucial for proper cell division. Dysregulation of cyclin B1 can lead to uncontrolled cell proliferation and is associated with various cancers, highlighting cyclin B1′s importance in cell cycle regulation and oncogenesis. Studying cyclin B1′s function provides insights into the mechanisms of cell division and potential therapeutic targets for cancer treatment. Cyclin B1 monoclonal antibody (GNS1) enables researchers to detect and analyze cyclin B1 expression and localization in different tissues and cell types, contributing to a better understanding of cyclin B1′s role in cellular processes. Cyclin B1 monoclonal antibody (GNS1) serves as a valuable tool in cell biology and cancer research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CCNB1/cyclin B1 Antibody (GNS1) References:

1. Oscillation of MPF is accompanied by periodic association between cdc25 and cdc2-cyclin B.  |  Jessus, C. and Beach, D. 1992. Cell. 68: 323-32. PMID: 1310257
2. Cyclin B1 and other cyclins as tumor antigens in immunosurveillance and immunotherapy of cancer.  |  Egloff, AM., et al. 2006. Cancer Res. 66: 6-9. PMID: 16397206
3. Cyclin B1 proteolysis via p38 MAPK signaling participates in G2 checkpoint elicited by arsenite.  |  Li, JP. and Yang, JL. 2007. J Cell Physiol. 212: 481-8. PMID: 17373649
4. Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation.  |  Gautier, J. and Maller, JL. 1991. EMBO J. 10: 177-82. PMID: 1824935
5. Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins.  |  Galaktionov, K. and Beach, D. 1991. Cell. 67: 1181-94. PMID: 1836978
6. Cyclin is a component of maturation-promoting factor from Xenopus.  |  Gautier, J., et al. 1990. Cell. 60: 487-94. PMID: 1967981
7. Control of M-phase by maturation-promoting factor.  |  Dorée, M. 1990. Curr Opin Cell Biol. 2: 269-73. PMID: 2141993
8. Direct activation of cdc2 with phosphatase: identification of p13suc1-sensitive and insensitive steps.  |  Jessus, C., et al. 1990. FEBS Lett. 266: 4-8. PMID: 2163887
9. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
10. Dominoes and clocks: the union of two views of the cell cycle.  |  Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
11. Cyclin B1/CDK1-regulated mitochondrial bioenergetics in cell cycle progression and tumor resistance.  |  Xie, B., et al. 2019. Cancer Lett. 443: 56-66. PMID: 30481564
12. Nuclear-cytoplasmic compartmentalization of cyclin B1-Cdk1 promotes robust timing of mitotic events.  |  Maryu, G. and Yang, Q. 2022. Cell Rep. 41: 111870. PMID: 36577372

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CCNB1/cyclin B1 Antibody (GNS1) has 1,250 citations in a variety of scientific publications.

Primary antibodies like Anti-CCNB1/cyclin B1 Antibody (GNS1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CCNB1/cyclin B1 Antibody (GNS1) Specifications

Alternate Product Name: cyclin B1

Product Clone Name: GNS1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: cyclin B1

Epitope Species: human

Product Reactivity/Detection: cyclin B1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1,250 citations in various scientific publications

Available Conjugates/Forms for CCNB1/cyclin B1 Antibody (GNS1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine, X: TransCruz Reagent