Cya A Antibody (9D4): sc-13581

Cya A Antibody (9D4) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Cya A protein of Bordetella pertussis origin by western blotting (WB) and immunoprecipitation (IP). Cya A (9D4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cya A protein, also known as adenylate cyclase toxin, plays a critical role in the pathogenesis of whooping cough by elevating intracellular cyclic adenosine monophosphate (cAMP) levels in host cells, leading to cytotoxic effects. This elevation in cAMP disrupts normal cellular signaling pathways, ultimately impairing immune responses and facilitating bacterial survival. The Cya A gene is part of a larger locus that encodes several proteins essential for function, including Cya C, which is necessary for Cya A activation, and Cya B, D, and E, which are involved in transport. Notably, Cya A interacts with the αMβ2 integrin (CD11b/CD18), predominantly found on immune cells such as neutrophils and macrophages, highlighting a role in evading the host′s innate immune response. Understanding Cya A mechanisms allows researchers to explore potential therapeutic targets for combating Bordetella pertussis infections.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cya A Antibody (9D4) References:

1. Adenylate cyclase toxin-hemolysin relevance for pertussis vaccines.  |  Sebo, P., et al. 2014. Expert Rev Vaccines. 13: 1215-27. PMID: 25090574
2. Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells.  |  Martín, C., et al. 2015. Sci Rep. 5: 13774. PMID: 26346097
3. Understanding the Mechanism of Translocation of Adenylate Cyclase Toxin across Biological Membranes.  |  Ostolaza, H., et al. 2017. Toxins (Basel). 9: PMID: 28934133
4. Structure-Function Relationships Underlying the Capacity of Bordetella Adenylate Cyclase Toxin to Disarm Host Phagocytes.  |  Novak, J., et al. 2017. Toxins (Basel). 9: PMID: 28946636
5. Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy.  |  Chenal, A. and Ladant, D. 2018. Toxins (Basel). 10: PMID: 30037010
6. Adenylate Cyclase Toxin Tinkering With Monocyte-Macrophage Differentiation.  |  Ahmad, JN. and Sebo, P. 2020. Front Immunol. 11: 2181. PMID: 33013916
7. Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Vaccine Development and Other Biotechnological Purposes.  |  Ladant, D. 2021. Toxins (Basel). 13: PMID: 33499260
8. The adenylate cyclase toxin RTX domain follows a series templated folding mechanism with implications for toxin activity.  |  Chen, G., et al. 2023. J Biol Chem. 299: 105150. PMID: 37567473
9. Adenylate cyclase toxin of Bordetella parapertussis disrupts the epithelial barrier granting the bacterial access to the intracellular space of epithelial cells.  |  Gorgojo, JP., et al. 2023. PLoS One. 18: e0291331. PMID: 38011105
10. Bordetella filamentous hemagglutinin and adenylate cyclase toxin interactions on the bacterial surface are consistent with FhaB-mediated delivery of ACT to phagocytic cells.  |  Nash, ZM., et al. 2024. mBio. 15: e0063224. PMID: 38534159

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cya A Antibody (9D4) has 5 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cya A Antibody (9D4) Specifications

Alternate Product Name: Cya A

Product Clone Name: 9D4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1156-1489

Epitope Target: Cya A

Epitope Species: Bordetella pertussis

Product Reactivity/Detection: Cya A

Species Reactivity/Detection: B. pertussis

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Cya A Antibody (9D4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Cya A antibody is Cya A Antibody (3D1): sc-13582.