Cya A Antibody (3D1): sc-13582

Cya A Antibody (3D1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Cya A protein of Bordetella pertussis origin by western blotting (WB) and immunoprecipitation (IP). Cya A (3D1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cya A protein, also known as adenylate cyclase toxin, plays a critical role in the pathogenesis of whooping cough by elevating intracellular cyclic adenosine monophosphate (cAMP) levels in host cells, leading to cytotoxic effects. This elevation disrupts normal cellular signaling pathways, which is crucial for the bacterium′s ability to evade the host′s immune response. The Cya A gene is part of a larger locus that encodes several proteins necessary for transport and activation, including Cya C, which is essential for converting the protoxin to its active form. Additionally, Cya A interacts with the αMβ2 integrin (CD11b/CD18), predominantly found on immune cells such as neutrophils and macrophages, thereby facilitating entry into these cells and contributing to the disruption of the innate immune response. Understanding Cya A′s function and interactions is vital for developing therapeutic strategies against Bordetella pertussis infections.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cya A Antibody (3D1) References:

1. Neutralizing antibodies to adenylate cyclase toxin promote phagocytosis of Bordetella pertussis by human neutrophils.  |  Weingart, CL., et al. 2000. Infect Immun. 68: 7152-5. PMID: 11083845
2. The adenylate cyclase toxin of Bordetella pertussis binds to target cells via the alpha(M)beta(2) integrin (CD11b/CD18).  |  Guermonprez, P., et al. 2001. J Exp Med. 193: 1035-44. PMID: 11342588
3. Enzymatic activity of adenylate cyclase toxin from Bordetella pertussis is not required for hemolysis.  |  Ehrmann, IE., et al. 1992. FEBS Lett. 304: 51-6. PMID: 1319923
4. Targeted mutations that ablate either the adenylate cyclase or hemolysin function of the bifunctional cyaA toxin of Bordetella pertussis abolish virulence.  |  Gross, MK., et al. 1992. Proc Natl Acad Sci U S A. 89: 4898-902. PMID: 1594590
5. High-level synthesis of active adenylate cyclase toxin of Bordetella pertussis in a reconstructed Escherichia coli system.  |  Sebo, P., et al. 1991. Gene. 104: 19-24. PMID: 1916273
6. Adenylate cyclase toxin promotes internalisation of integrins and raft components and decreases macrophage adhesion capacity.  |  Martín, C., et al. 2011. PLoS One. 6: e17383. PMID: 21383852
7. Adenylate cyclase toxin-mediated delivery of the S1 subunit of pertussis toxin into mammalian cells.  |  Iwaki, M. and Konda, T. 2016. Pathog Dis. 74: ftv110. PMID: 26607401
8. Phospholipase A activity of adenylate cyclase toxin mediates translocation of its adenylate cyclase domain.  |  González-Bullón, D., et al. 2017. Proc Natl Acad Sci U S A. 114: E6784-E6793. PMID: 28760979
9. Membrane Permeabilization by Bordetella Adenylate Cyclase Toxin Involves Pores of Tunable Size.  |  González-Bullón, D., et al. 2019. Biomolecules. 9: PMID: 31083482
10. Bordetella Adenylate Cyclase Toxin Inhibits Monocyte-to-Macrophage Transition and Dedifferentiates Human Alveolar Macrophages into Monocyte-like Cells.  |  Ahmad, JN., et al. 2019. mBio. 10: PMID: 31551332
11. Membrane Activity and Channel Formation of the Adenylate Cyclase Toxin (CyaA) of Bordetella pertussis in Lipid Bilayer Membranes.  |  Knapp, O. and Benz, R. 2020. Toxins (Basel). 12: PMID: 32164365
12. Bordetella pertussis adenylate cyclase toxin: proCyaA and CyaC proteins synthesised separately in Escherichia coli produce active toxin in vitro.  |  Westrop, GD., et al. 1996. Gene. 180: 91-9. PMID: 8973351

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cya A Antibody (3D1) has 74 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cya A Antibody (3D1) Specifications

Alternate Product Name: Cya A

Product Clone Name: 3D1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-400

Epitope Target: Cya A

Epitope Species: Bordetella pertussis

Product Reactivity/Detection: Cya A

Species Reactivity/Detection: B. pertussis

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 74 citations in various scientific publications

Available Conjugates/Forms for Cya A Antibody (3D1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Cya A antibody is Cya A Antibody (9D4): sc-13581.