CXCR-4 Antibody (4G10): sc-53534

CXCR-4 Antibody (4G10) is a mouse monoclonal IgG1 kappa light chain antibody raised against the N-terminus amino acids of CXCR-4 of human origin, reactive with mouse, rat, and human species, and validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and immunohistochemistry. CXCR-4, also known as CD184 or Fusin, is a seven-transmembrane G protein-coupled receptor that resides primarily on the cell surface. CXCR-4 is highly expressed in various tissues and plays a critical role in cellular signaling, particularly in immune and developmental processes. CXCR-4 interacts with stromal cell-derived factor-1 (SDF-1) to mediate chemotactic migration of immune cells such as T cells and monocytes. CXCR-4′s cell surface location enables efficient detection and response to extracellular signals, guiding cell movement during immune responses, tissue repair, and development. CXCR-4′s localization to the plasma membrane facilitates a role as a major co-receptor for T-tropic HIV-1, aiding in viral entry into host cells, while SDF-1 acts as an inhibitor of HIV-1 infection, highlighting significance in antiviral research. CXCR-4 is implicated in cancer progression, as dysregulated expression and signaling are associated with metastasis and tumor cell survival. Anti-CXCR-4 antibody (4G10) is available in non-conjugated form and as various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® options, offering researchers a versatile tool for exploring CXCR-4′s critical roles and cellular dynamics in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CXCR-4 Antibody (4G10) References:

1. Pillars article: HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996. 272: 872-877.  |  Feng, Y., et al. 2011. J Immunol. 186: 6076-81. PMID: 21597040
2. CXC chemokines bind to unique sets of selectivity determinants that can function independently and are broadly distributed on multiple domains of human interleukin-8 receptor B. Determinants of high affinity binding and receptor activation are distinct.  |  Ahuja, SK., et al. 1996. J Biol Chem. 271: 225-32. PMID: 8550564
3. Rapid mobilization of hematopoietic progenitor cells in rhesus monkeys by a single intravenous injection of interleukin-8.  |  Laterveer, L., et al. 1996. Blood. 87: 781-8. PMID: 8555503
4. Identification of a major co-receptor for primary isolates of HIV-1.  |  Deng, H., et al. 1996. Nature. 381: 661-6. PMID: 8649511
5. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry.  |  Bleul, CC., et al. 1996. Nature. 382: 829-33. PMID: 8752280
6. Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection.  |  Liu, R., et al. 1996. Cell. 86: 367-77. PMID: 8756719
7. Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1.  |  Nagasawa, T., et al. 1996. Nature. 382: 635-8. PMID: 8757135

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CXCR-4 Antibody (4G10) has 90 citations in a variety of scientific publications.

Primary antibodies like Anti-CXCR-4 Antibody (4G10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CXCR-4 Antibody (4G10) Specifications

Alternate Product Name: CXCR-4

Product Clone Name: 4G10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-38

Epitope Target: CXCR-4

Epitope Species: human

Product Reactivity/Detection: CXCR-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 90 citations in various scientific publications

Available Conjugates/Forms for CXCR-4 Antibody (4G10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CXCR-4 antibody is CXCR-4 Antibody (12G5): sc-12764.