CUG-BP1/2 Antibody (F-1): sc-166198
- CUG-BP1/2 Antibody (F-1) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 9-194 mapping near the N-terminus of CUG-BP1 of human origin
- recommended for detection of CUG-BP1 and CUG-BP2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- See CUG-BP1 (3B1): sc-20003 for CUG-BP1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CUG-BP1/2 Antibody (F-1) for WB applications. These reagents are now offered in bundles with CUG-BP1/2 Antibody (F-1) (see ordering information below).
CUG-BP1/2 Antibody (F-1) is a mouse monoclonal IgG1 antibody that detects CUG-BP1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CUG-BP1 plays a crucial role in the regulation of RNA splicing and translation, particularly in the context of myotonic dystrophy, where binding activity is altered due to the expansion of (CTG)n repeats in the myotonin protein kinase gene (DMPK). CUG-BP1 is primarily located in the nucleus, where CUG-BP1 interacts with RNA to modulate the expression of various target genes, including the C/EBPb transcription factor. Nuclear localization of CUG-BP1 is vital for function, as nuclear presence allows for precise regulation of mRNA processing and translation, which is essential for normal cellular function and differentiation. In myotonic dystrophy, dysregulation of CUG-BP1 leads to a decrease in the translation of key proteins such as p21, which is critical for cell cycle regulation. By utilizing CUG-BP1/2 (F-1) antibody, researchers can gain insights into the molecular mechanisms underlying myotonic dystrophy and explore potential therapeutic targets for this debilitating condition.
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CUG-BP1/2 Antibody (F-1) References:
- Transgenic mice expressing CUG-BP1 reproduce splicing mis-regulation observed in myotonic dystrophy. | Ho, TH., et al. 2005. Hum Mol Genet. 14: 1539-47. PMID: 15843400
- CUG-BP binds to RNA substrates and recruits PARN deadenylase. | Moraes, KC., et al. 2006. RNA. 12: 1084-91. PMID: 16601207
- CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. | Marquis, J., et al. 2006. Biochem J. 400: 291-301. PMID: 16938098
- Quantitative analysis of CUG-BP1 binding to RNA repeats. | Mori, D., et al. 2008. J Biochem. 143: 377-83. PMID: 18039683
- Identification of CUG-BP1/EDEN-BP target mRNAs in Xenopus tropicalis. | Graindorge, A., et al. 2008. Nucleic Acids Res. 36: 1861-70. PMID: 18267972
- Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM3. | Tsuda, K., et al. 2009. Nucleic Acids Res. 37: 5151-66. PMID: 19553194
- The importance of CELF control: molecular and biological roles of the CUG-BP, Elav-like family of RNA-binding proteins. | Dasgupta, T. and Ladd, AN. 2012. Wiley Interdiscip Rev RNA. 3: 104-21. PMID: 22180311
- The RNA-binding protein CUG-BP1 increases survivin expression in oesophageal cancer cells through enhanced mRNA stability. | Chang, ET., et al. 2012. Biochem J. 446: 113-23. PMID: 22646166
- CUG-BP, Elav-like family (CELF)-mediated alternative splicing regulation in the brain during health and disease. | Ladd, AN. 2013. Mol Cell Neurosci. 56: 456-64. PMID: 23247071
- CUG-BP1 regulates RyR1 ASI alternative splicing in skeletal muscle atrophy. | Tang, Y., et al. 2015. Sci Rep. 5: 16083. PMID: 26531141
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CUG-BP1/2 Antibody (F-1) | sc-166198 | 200 µg/ml | $322.00 | |||
CUG-BP1/2 Antibody (F-1): m-IgG Fc BP-HRP Bundle | sc-537455 | 200 µg Ab; 10 µg BP | $361.00 | |||
CUG-BP1/2 Antibody (F-1): m-IgGκ BP-HRP Bundle | sc-534727 | 200 µg Ab; 40 µg BP | $361.00 | |||
CUG-BP1/2 Antibody (F-1): m-IgG1 BP-HRP Bundle | sc-545191 | 200 µg Ab; 20 µg BP | $361.00 |