CUG-BP1/2 Antibody (E-10): sc-166096

CUG-BP1/2 Antibody (E-10) is a mouse monoclonal IgG2b antibody that detects CUG-BP1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CUG-BP1 plays a crucial role in RNA splicing and translation regulation, particularly in myotonic dystrophy, a neuromuscular disorder characterized by a (CTG)n repeat expansion in the myotonin protein kinase gene (DMPK). CUG-BP1 is primarily located in the nucleus, where binding to CUG repeat sequences influences target gene expression. Nuclear localization of CUG-BP1 enables precise regulation of mRNA processing and translation, essential for maintaining normal cellular function and response to differentiation signals. In normal cells, CUG-BP1 enhances p21 protein translation, a critical cell cycle regulator, by binding to specific sequences in p21 mRNA. However, in myotonic dystrophy, altered binding dynamics of CUG-BP1 due to expanded CUG repeats lead to reduced levels of p21 and other cell cycle regulation proteins, contributing to disease pathology. CUG-BP1/2 (E-10) antibody serves as an invaluable tool for researchers studying molecular mechanisms underlying myotonic dystrophy and broader implications of RNA-binding proteins in gene expression regulation.

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CUG-BP1/2 Antibody (E-10) References:

1. Isolation and expression of Napor/CUG-BP2 in embryo development.  |  Choi, DK., et al. 2003. Biochem Biophys Res Commun. 305: 448-54. PMID: 12763013
2. Epidermal growth factor receptor stimulation activates the RNA binding protein CUG-BP1 and increases expression of C/EBPbeta-LIP in mammary epithelial cells.  |  Baldwin, BR., et al. 2004. Mol Cell Biol. 24: 3682-91. PMID: 15082764
3. Protein expression is increased by a class III AU-rich element and tethered CUG-BP1.  |  Barreau, C., et al. 2006. Biochem Biophys Res Commun. 347: 723-30. PMID: 16843434
4. Inactivation of CUG-BP1/CELF1 causes growth, viability, and spermatogenesis defects in mice.  |  Kress, C., et al. 2007. Mol Cell Biol. 27: 1146-57. PMID: 17130239
5. Quantitative analysis of CUG-BP1 binding to RNA repeats.  |  Mori, D., et al. 2008. J Biochem. 143: 377-83. PMID: 18039683
6. SRp20 and CUG-BP1 modulate insulin receptor exon 11 alternative splicing.  |  Sen, S., et al. 2009. Mol Cell Biol. 29: 871-80. PMID: 19047369
7. Known turnover and translation regulatory RNA-binding proteins interact with the 3' UTR of SECIS-binding protein 2.  |  Bubenik, JL., et al. 2009. RNA Biol. 6: 73-83. PMID: 19106619
8. Analysis of MTMR1 expression and correlation with muscle pathological features in juvenile/adult onset myotonic dystrophy type 1 (DM1) and in myotonic dystrophy type 2 (DM2).  |  Santoro, M., et al. 2010. Exp Mol Pathol. 89: 158-68. PMID: 20685272
9. The RNA-binding protein CUG-BP1 increases survivin expression in oesophageal cancer cells through enhanced mRNA stability.  |  Chang, ET., et al. 2012. Biochem J. 446: 113-23. PMID: 22646166
10. CUG-BP1 regulates RyR1 ASI alternative splicing in skeletal muscle atrophy.  |  Tang, Y., et al. 2015. Sci Rep. 5: 16083. PMID: 26531141

The preferred CUG-BP1 antibody is CUG-BP1 Antibody (3B1): sc-20003.