CtIP Antibody (D-4): sc-271339

CtIP Antibody (D-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects CtIP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CtIP antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CtIP protein, also known as RBBP8, plays a crucial role in DNA repair and cell cycle regulation, particularly in double-strand break repair. CtIP is primarily located in the nucleus, where CtIP interacts with key proteins involved in homologous recombination repair pathway, such as BRCA1. This interaction helps maintain genomic stability and prevent tumorigenesis. CtIP binding to BRCA1 through BRCT repeats is essential for regulating transcription of genes involved in DNA damage response, including p21 promoter, which is critical for cell cycle arrest following DNA damage. Through modulation of BRCA1 activity, CtIP influences cellular response to DNA damage, highlighting CtIP′s importance in cancer biology and therapeutic strategies.

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CtIP Antibody (D-4) References:

1. Binding of CtIP to the BRCT repeats of BRCA1 involved in the transcription regulation of p21 is disrupted upon DNA damage.  |  Li, S., et al. 1999. J Biol Chem. 274: 11334-8. PMID: 10196224
2. Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1.  |  Furusawa, T., et al. 1999. Mol Cell Biol. 19: 8581-90. PMID: 10567582
3. Emergence of antitumor cytolytic T cells is associated with maintenance of hematologic remission in children with acute myeloid leukemia.  |  Montagna, D., et al. 2006. Blood. 108: 3843-50. PMID: 16868250
4. The CTIP-mediated repair of TNF-α-induced DNA double-strand break was impaired by miR-130b in cervical cancer cell.  |  Yang, L., et al. 2019. Cell Biochem Funct. 37: 534-544. PMID: 31418900
5. SUMOylation mediates CtIP's functions in DNA end resection and replication fork protection.  |  Locke, AJ., et al. 2021. Nucleic Acids Res. 49: 928-953. PMID: 33406258
6. CtIP-dependent nascent RNA expression flanking DNA breaks guides the choice of DNA repair pathway.  |  Gómez-Cabello, D., et al. 2022. Nat Commun. 13: 5303. PMID: 36085345
7. The CtBP binding domain in the adenovirus E1A protein controls CR1-dependent transactivation.  |  Sollerbrant, K., et al. 1996. Nucleic Acids Res. 24: 2578-84. PMID: 8692699
8. Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular protein is disrupted by E1A through a conserved PLDLS motif.  |  Schaeper, U., et al. 1998. J Biol Chem. 273: 8549-52. PMID: 9535825
9. Two mechanisms in the action of repressor deltaEF1: binding site competition with an activator and active repression.  |  Sekido, R., et al. 1997. Genes Cells. 2: 771-83. PMID: 9544704
10. Cloning and characterization of mCtBP2, a co-repressor that associates with basic Krüppel-like factor and other mammalian transcriptional regulators.  |  Turner, J. and Crossley, M. 1998. EMBO J. 17: 5129-40. PMID: 9724649
11. The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression.  |  Yu, X., et al. 1998. J Biol Chem. 273: 25388-92. PMID: 9738006
12. Characterization of a carboxy-terminal BRCA1 interacting protein.  |  Wong, AK., et al. 1998. Oncogene. 17: 2279-85. PMID: 9811458

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CtIP Antibody (D-4) has 49 citations in a variety of scientific publications.

Primary antibodies like Anti-CtIP Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CtIP Antibody (D-4) Specifications

Alternate Product Name: RBBP8

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 835-865

Epitope Target: CtIP

Epitope Species: human

Product Reactivity/Detection: CtIP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 49 citations in various scientific publications

Available Conjugates/Forms for CtIP Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin