CTF18 Antibody (F-1): sc-374632
- CTF18 Antibody (F-1) is a mouse monoclonal IgG2a κ CTF18 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 887-975 mapping at the C-terminal of CTF18 of human origin
- CTF18 Antibody (F-1) is recommended for detection of CTF18 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CTF18 Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for CTF18 Antibody (F-1) for WB applications. This reagent is now offered in a bundle with CTF18 Antibody (F-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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CTF18 Antibody (F-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects CTF18 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CTF18 Antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CTF18, also known as chromosome transmission fidelity factor 18 homolog, is a crucial 975 amino acid protein encoded by the human gene CHTF18. CTF18 plays a significant role in maintaining genomic stability by participating in the DNA replication block checkpoint, functioning redundantly with Rad24. CTF18 is part of a larger replication factor C (RFC) complex, which is a seven-subunit structure that includes four small RFC subunits along with CTF18, DCC1, and CTF8. This complex is essential for loading the replication clamp, proliferating cell nuclear antigen (PCNA), onto DNA, thereby facilitating DNA replication and repair processes. Notably, the regulated unloading of PCNA during DNA replication′s progression and termination is not a function attributed to the CTF18-RFC complex, highlighting CTF18′s specialized role in ensuring the fidelity of chromosome transmission during cell division.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CTF18 Antibody (F-1) References:
- Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway. | Naiki, T., et al. 2001. Mol Cell Biol. 21: 5838-45. PMID: 11486023
- A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein. | Ohta, S., et al. 2002. J Biol Chem. 277: 40362-7. PMID: 12171929
- Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment. | Merkle, CJ., et al. 2003. J Biol Chem. 278: 30051-6. PMID: 12766176
- The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA. | Bermudez, VP., et al. 2003. Proc Natl Acad Sci U S A. 100: 10237-42. PMID: 12930902
- Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability. | Kanellis, P., et al. 2003. Curr Biol. 13: 1583-95. PMID: 13678589
- The INO80 chromatin remodeling complex functions in sister chromatid cohesion. | Ogiwara, H., et al. 2007. Cell Cycle. 6: 1090-5. PMID: 17471029
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTF18 Antibody (F-1) | sc-374632 | 200 µg/ml | $322.00 | |||
CTF18 Antibody (F-1): m-IgGκ BP-HRP Bundle | sc-522797 | 200 µg Ab, 40 µg BP | $361.00 | |||
CTF18 Antibody (F-1) AC | sc-374632 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CTF18 Antibody (F-1) HRP | sc-374632 HRP | 200 µg/ml | $322.00 | |||
CTF18 Antibody (F-1) FITC | sc-374632 FITC | 200 µg/ml | $336.00 | |||
CTF18 Antibody (F-1) PE | sc-374632 PE | 200 µg/ml | $349.00 | |||
CTF18 Antibody (F-1) Alexa Fluor® 488 | sc-374632 AF488 | 200 µg/ml | $364.00 | |||
CTF18 Antibody (F-1) Alexa Fluor® 546 | sc-374632 AF546 | 200 µg/ml | $364.00 | |||
CTF18 Antibody (F-1) Alexa Fluor® 594 | sc-374632 AF594 | 200 µg/ml | $364.00 | |||
CTF18 Antibody (F-1) Alexa Fluor® 647 | sc-374632 AF647 | 200 µg/ml | $364.00 | |||
CTF18 Antibody (F-1) Alexa Fluor® 680 | sc-374632 AF680 | 200 µg/ml | $364.00 | |||
CTF18 Antibody (F-1) Alexa Fluor® 790 | sc-374632 AF790 | 200 µg/ml | $364.00 |