CTCF Antibody (B-5): sc-271514
- CTCF Antibody (B-5) is a mouse monoclonal IgG1 κ, cited in 13 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 643-687 near the C-terminus of CTCF of human origin
- Anti-CTCF Antibody (B-5) is recommended for detection of CTCF of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- TransCruz reagent for ChIP application (sc-271514 X, 200 µg/0.1 ml)
- Anti-CTCF Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CTCF Antibody (B-5) for WB applications. These reagents are now offered in bundles with CTCF Antibody (B-5) (see ordering information below).
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CTCF Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CTCF from mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CTCF Antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CTCF, or CCCTC-binding factor, is a critical zinc finger transcription factor that plays a vital role in regulating gene expression by binding to long and diverse DNA sequences. CTCF′s unique structure, characterized by 11 zinc fingers—10 of the C2H2 class and 1 of the C2HC class—enables binding to a wide array of genomic elements, influencing the transcription of numerous genes. This structural complexity is essential for functioning as a transcriptional repressor of the c-myc gene and as a regulator of lysozyme gene expression. Additionally, CTCF′s interaction with the promoter region of the amyloid beta-protein precursor (APP) gene is crucial for activating APP transcription, which is significant for synapse formation during neuronal development. CTCF is predominantly localized in the nucleus and is expressed ubiquitously across various tissues. Expression is tightly regulated, particularly during terminal differentiation, where negative regulation occurs through differential phosphorylation and reductions in both CTCF mRNA and protein levels.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CTCF Antibody (B-5) References:
- The ChAHP Complex Counteracts Chromatin Looping at CTCF Sites that Emerged from SINE Expansions in Mouse. | Kaaij, LJT., et al. 2019. Cell. 178: 1437-1451.e14. PMID: 31491387
- CTCF and cellular heterogeneity. | Ren, G. and Zhao, K. 2019. Cell Biosci. 9: 83. PMID: 31632638
- CTCF and CTCFL in cancer. | Debaugny, RE. and Skok, JA. 2020. Curr Opin Genet Dev. 61: 44-52. PMID: 32334335
- CTCF organizes inter-A compartment interactions through RYBP-dependent phase separation. | Wei, C., et al. 2022. Cell Res. 32: 744-760. PMID: 35768498
- BAP18 facilitates CTCF-mediated chromatin accessible to regulate enhancer activity in breast cancer. | Sun, G., et al. 2023. Cell Death Differ. 30: 1260-1278. PMID: 36828916
- CTCF and Its Multi-Partner Network for Chromatin Regulation. | Del Moral-Morales, A., et al. 2023. Cells. 12: PMID: 37408191
- Chromatin architectural factor CTCF is essential for progesterone-dependent uterine maturation. | Hewitt, SC., et al. 2023. FASEB J. 37: e23103. PMID: 37489832
- Regulation of CTCF loop formation during pancreatic cell differentiation. | Lyu, X., et al. 2023. Nat Commun. 14: 6314. PMID: 37813869
- Dissection of a CTCF topological boundary uncovers principles of enhancer-oncogene regulation. | Kim, KL., et al. 2024. Mol Cell. 84: 1365-1376.e7. PMID: 38452764
- ADNP modulates SINE B2-derived CTCF-binding sites during blastocyst formation in mice. | Wang, W., et al. 2024. Genes Dev. 38: 168-188. PMID: 38479840
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTCF Antibody (B-5) | sc-271514 | 200 µg/ml | $322.00 | |||
CTCF Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-529198 | 200 µg Ab; 10 µg BP | $361.00 | |||
CTCF Antibody (B-5): m-IgGκ BP-HRP Bundle | sc-521965 | 200 µg Ab, 40 µg BP | $361.00 | |||
CTCF Antibody (B-5): m-IgG1 BP-HRP Bundle | sc-543380 | 200 µg Ab; 20 µg BP | $361.00 | |||
CTCF Antibody (B-5) X | sc-271514 X | 200 µg/0.1 ml | $322.00 | |||
CTCF Antibody (B-5) AC | sc-271514 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CTCF Antibody (B-5) HRP | sc-271514 HRP | 200 µg/ml | $322.00 | |||
CTCF Antibody (B-5) FITC | sc-271514 FITC | 200 µg/ml | $336.00 | |||
CTCF Antibody (B-5) PE | sc-271514 PE | 200 µg/ml | $349.00 | |||
CTCF Antibody (B-5) Alexa Fluor® 488 | sc-271514 AF488 | 200 µg/ml | $364.00 | |||
CTCF Antibody (B-5) Alexa Fluor® 546 | sc-271514 AF546 | 200 µg/ml | $364.00 | |||
CTCF Antibody (B-5) Alexa Fluor® 594 | sc-271514 AF594 | 200 µg/ml | $364.00 | |||
CTCF Antibody (B-5) Alexa Fluor® 647 | sc-271514 AF647 | 200 µg/ml | $364.00 | |||
CTCF Antibody (B-5) Alexa Fluor® 680 | sc-271514 AF680 | 200 µg/ml | $364.00 | |||
CTCF Antibody (B-5) Alexa Fluor® 790 | sc-271514 AF790 | 200 µg/ml | $364.00 | |||
CTCF (B-5) Neutralizing Peptide | sc-271514 P | 100 µg/0.5 ml | $69.00 |