CTCF Antibody (G-8): sc-271474

CTCF Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects CTCF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-CTCF antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CTCF, a member of the zinc finger transcription factor family, plays a crucial role in gene regulation by recognizing long and diverse DNA sequences, which is essential for maintaining proper gene expression patterns. CTCF′s unique structure, characterized by 11 zinc finger motifs—10 of the C2H2 class and 1 of the C2HC class—enables binding to specific DNA regions, influencing transcription of numerous genes. CTCF acts as a repressor of the c-myc gene and regulates expression of the lysozyme gene, while also interacting with the promoter region of the amyloid beta-protein precursor (APP) gene to enhance transcription. This interaction promotes synapse formation between primary neurons during development, highlighting CTCF′s role in neurodevelopmental processes. CTCF is ubiquitously expressed and localized in the nucleus, where activity is modulated by post-translational modifications such as phosphorylation, which can negatively regulate function during terminal differentiation.

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CTCF Antibody (G-8) References:

1. Differential expression and phosphorylation of CTCF, a c-myc transcriptional regulator, during differentiation of human myeloid cells.  |  Delgado, MD., et al. 1999. FEBS Lett. 444: 5-10. PMID: 10037138
2. The protein CTCF is required for the enhancer blocking activity of vertebrate insulators.  |  Bell, AC., et al. 1999. Cell. 98: 387-96. PMID: 10458613
3. Negative transcriptional regulation mediated by thyroid hormone response element 144 requires binding of the multivalent factor CTCF to a novel target DNA sequence.  |  Awad, TA., et al. 1999. J Biol Chem. 274: 27092-8. PMID: 10480923
4. CTCF is essential for up-regulating expression from the amyloid precursor protein promoter during differentiation of primary hippocampal neurons.  |  Yang, Y., et al. 1999. J Neurochem. 73: 2286-98. PMID: 10582586
5. CTCF-Mediated Human 3D Genome Architecture Reveals Chromatin Topology for Transcription.  |  Tang, Z., et al. 2015. Cell. 163: 1611-27. PMID: 26686651
6. Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging.  |  Gabriele, M., et al. 2022. Science. 376: 496-501. PMID: 35420890
7. Enhancer-promoter interactions can bypass CTCF-mediated boundaries and contribute to phenotypic robustness.  |  Chakraborty, S., et al. 2023. Nat Genet. 55: 280-290. PMID: 36717694
8. The impact of DNA methylation on CTCF-mediated 3D genome organization.  |  Monteagudo-Sánchez, A., et al. 2024. Nat Struct Mol Biol. 31: 404-412. PMID: 38499830
9. CTCF mutation at R567 causes developmental disorders via 3D genome rearrangement and abnormal neurodevelopment.  |  Zhang, J., et al. 2024. Nat Commun. 15: 5524. PMID: 38951485
10. CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple forms.  |  Klenova, EM., et al. 1993. Mol Cell Biol. 13: 7612-24. PMID: 8246978
11. An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes.  |  Filippova, GN., et al. 1996. Mol Cell Biol. 16: 2802-13. PMID: 8649389
12. The zinc finger protein CTCF binds to the APBbeta domain of the amyloid beta-protein precursor promoter. Evidence for a role in transcriptional activation.  |  Vostrov, AA. and Quitschke, WW. 1997. J Biol Chem. 272: 33353-9. PMID: 9407128

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CTCF Antibody (G-8) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-CTCF Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CTCF Antibody (G-8) Specifications

Alternate Product Name: CCCTC-binding factor (zinc finger protein)

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-280

Epitope Target: CTCF

Epitope Species: human

Product Reactivity/Detection: CTCF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for CTCF Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent