CtBP1 Antibody (G-6): sc-398945

CtBP1 Antibody (G-6) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect C-terminal binding protein 1 (CtBP1), also known as E1A binding protein p120, across multiple species including mouse, rat, and human. anti-CtBP1 antibody (G-6) targets an internal epitope spanning amino acids 349-369 of human CtBP1, ensuring precise recognition in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CtBP1 is predominantly localized in the nucleus, where CtBP1 functions as a crucial transcriptional corepressor by interacting with various transcription factors and regulatory proteins to modulate gene expression. This localization is essential for its role in regulating processes such as embryonic development, cell differentiation, and cellular response to DNA damage, thereby maintaining cellular homeostasis and preventing oncogenic transformations. CtBP1 operates in concert with CtBP2 homolog and interacts with proteins like CtIP and BRCA1, highlighting CtBP1′s significance in complex regulatory networks involved in cancer biology and gene silencing. CtBP1′s ability to shuttle between nucleus and cytoplasm under specific cellular conditions underscores CtBP1′s dynamic role in cellular regulation. Additionally, CtBP1′s involvement in repressing p21 promoter in response to DNA damage emphasizes CtBP1′s importance in cell cycle control and apoptosis. CtBP1 monoclonal antibody (G-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatile options for diverse experimental needs. anti-CtBP1 antibody (G-6) is an indispensable tool for researchers investigating intricate mechanisms of transcriptional repression, protein-protein interactions, and molecular pathways underlying development and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CtBP1 Antibody (G-6) References:

1. Binding of CtIP to the BRCT repeats of BRCA1 involved in the transcription regulation of p21 is disrupted upon DNA damage.  |  Li, S., et al. 1999. J Biol Chem. 274: 11334-8. PMID: 10196224
2. Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1.  |  Furusawa, T., et al. 1999. Mol Cell Biol. 19: 8581-90. PMID: 10567582
3. The effect of CtBP1 binding on the structure of the C-terminal region of adenovirus 12 early region 1A.  |  Molloy, DP., et al. 2007. Virology. 363: 342-56. PMID: 17335865
4. The CtBP binding domain in the adenovirus E1A protein controls CR1-dependent transactivation.  |  Sollerbrant, K., et al. 1996. Nucleic Acids Res. 24: 2578-84. PMID: 8692699
5. Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular protein is disrupted by E1A through a conserved PLDLS motif.  |  Schaeper, U., et al. 1998. J Biol Chem. 273: 8549-52. PMID: 9535825
6. Two mechanisms in the action of repressor deltaEF1: binding site competition with an activator and active repression.  |  Sekido, R., et al. 1997. Genes Cells. 2: 771-83. PMID: 9544704
7. Cloning and characterization of mCtBP2, a co-repressor that associates with basic Krüppel-like factor and other mammalian transcriptional regulators.  |  Turner, J. and Crossley, M. 1998. EMBO J. 17: 5129-40. PMID: 9724649
8. The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression.  |  Yu, X., et al. 1998. J Biol Chem. 273: 25388-92. PMID: 9738006
9. Characterization of a carboxy-terminal BRCA1 interacting protein.  |  Wong, AK., et al. 1998. Oncogene. 17: 2279-85. PMID: 9811458

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CtBP1 Antibody (G-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CtBP1 Antibody (G-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CtBP1 Antibody (G-6) Specifications

Alternate Product Name: CtBP1

Product Clone Name: G-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 349-369

Epitope Target: CtBP1

Epitope Species: human

Product Reactivity/Detection: CtBP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CtBP1 Antibody (G-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred CtBP antibody is CtBP Antibody (E-12): sc-17759.