CtBP Antibody (E-12): sc-17759
- CtBP Antibody (E-12) is a mouse monoclonal IgG1 κ CtBP antibody, cited in 42 publications, provided at 200 µg/ml
- raised against amino acids 1-440 of CtBP of human origin
- CtBP Antibody (E-12) is recommended for detection of CtBP1 and CtBP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CtBP Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CtBP Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with CtBP Antibody (E-12) (see ordering information below).
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CtBP Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects CtBP protein of mouse, rat, and human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). CtBP (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. CtBP1 monoclonal antibody (E-12) recognizes a key cellular phosphoprotein that functions as a transcriptional corepressor, regulating gene expression and maintaining cellular homeostasis. CtBP1 (E-12) antibody targets a protein that interacts with δEF1, a zinc finger-homeodomain protein, enhancing transcriptional silencing during skeletal and T-cell development. CtBP1 forms complexes with CtIP, a protein binding to BRCT repeats of breast cancer gene BRCA1, influencing BRCA1′s activity. CtBP1 inhibits transactivation of p21 promoter, a critical regulator of cell cycle and DNA damage response, demonstrating significance in cellular stress responses and potential cancer biology implications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CtBP Antibody (E-12) References:
- Binding of CtIP to the BRCT repeats of BRCA1 involved in the transcription regulation of p21 is disrupted upon DNA damage. | Li, S., et al. 1999. J Biol Chem. 274: 11334-8. PMID: 10196224
- Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1. | Furusawa, T., et al. 1999. Mol Cell Biol. 19: 8581-90. PMID: 10567582
- A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP. | Pagan, JK., et al. 2007. J Biol Chem. 282: 15248-57. PMID: 17379597
- CtBP- an emerging oncogene and novel small molecule drug target: Advances in the understanding of its oncogenic action and identification of therapeutic inhibitors. | Dcona, MM., et al. 2017. Cancer Biol Ther. 18: 379-391. PMID: 28532298
- Inhibition of the CtBP complex and FBXO11 enhances MHC class II expression and anti-cancer immune responses. | Chan, KL., et al. 2022. Cancer Cell. 40: 1190-1206.e9. PMID: 36179686
- Metabolic modulation of CtBP dimeric status impacts the repression of DNA damage repair genes and the platinum sensitivity of ovarian cancer. | Li, J., et al. 2023. Int J Biol Sci. 19: 2081-2096. PMID: 37151877
- The CtBP binding domain in the adenovirus E1A protein controls CR1-dependent transactivation. | Sollerbrant, K., et al. 1996. Nucleic Acids Res. 24: 2578-84. PMID: 8692699
- Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular protein is disrupted by E1A through a conserved PLDLS motif. | Schaeper, U., et al. 1998. J Biol Chem. 273: 8549-52. PMID: 9535825
- Two mechanisms in the action of repressor deltaEF1: binding site competition with an activator and active repression. | Sekido, R., et al. 1997. Genes Cells. 2: 771-83. PMID: 9544704
- Cloning and characterization of mCtBP2, a co-repressor that associates with basic Krüppel-like factor and other mammalian transcriptional regulators. | Turner, J. and Crossley, M. 1998. EMBO J. 17: 5129-40. PMID: 9724649
- The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression. | Yu, X., et al. 1998. J Biol Chem. 273: 25388-92. PMID: 9738006
- Characterization of a carboxy-terminal BRCA1 interacting protein. | Wong, AK., et al. 1998. Oncogene. 17: 2279-85. PMID: 9811458
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CtBP Antibody (E-12) | sc-17759 | 200 µg/ml | $322.00 | |||
CtBP Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-520639 | 200 µg Ab, 40 µg BP | $361.00 | |||
CtBP Antibody (E-12): m-IgG1 BP-HRP Bundle | sc-533468 | 200 µg Ab; 20 µg BP | $361.00 | |||
CtBP Antibody (E-12) AC | sc-17759 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CtBP Antibody (E-12) HRP | sc-17759 HRP | 200 µg/ml | $322.00 | |||
CtBP Antibody (E-12) FITC | sc-17759 FITC | 200 µg/ml | $336.00 | |||
CtBP Antibody (E-12) PE | sc-17759 PE | 200 µg/ml | $349.00 | |||
CtBP Antibody (E-12) Alexa Fluor® 488 | sc-17759 AF488 | 200 µg/ml | $364.00 | |||
CtBP Antibody (E-12) Alexa Fluor® 546 | sc-17759 AF546 | 200 µg/ml | $364.00 | |||
CtBP Antibody (E-12) Alexa Fluor® 594 | sc-17759 AF594 | 200 µg/ml | $364.00 | |||
CtBP Antibody (E-12) Alexa Fluor® 647 | sc-17759 AF647 | 200 µg/ml | $364.00 | |||
CtBP Antibody (E-12) Alexa Fluor® 680 | sc-17759 AF680 | 200 µg/ml | $364.00 | |||
CtBP Antibody (E-12) Alexa Fluor® 790 | sc-17759 AF790 | 200 µg/ml | $364.00 |