CtBP Antibody (E-12): sc-17759

CtBP Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects CtBP protein of mouse, rat, and human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). CtBP (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. CtBP1 monoclonal antibody (E-12) recognizes a key cellular phosphoprotein that functions as a transcriptional corepressor, regulating gene expression and maintaining cellular homeostasis. CtBP1 (E-12) antibody targets a protein that interacts with δEF1, a zinc finger-homeodomain protein, enhancing transcriptional silencing during skeletal and T-cell development. CtBP1 forms complexes with CtIP, a protein binding to BRCT repeats of breast cancer gene BRCA1, influencing BRCA1′s activity. CtBP1 inhibits transactivation of p21 promoter, a critical regulator of cell cycle and DNA damage response, demonstrating significance in cellular stress responses and potential cancer biology implications.

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CtBP Antibody (E-12) References:

1. Binding of CtIP to the BRCT repeats of BRCA1 involved in the transcription regulation of p21 is disrupted upon DNA damage.  |  Li, S., et al. 1999. J Biol Chem. 274: 11334-8. PMID: 10196224
2. Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1.  |  Furusawa, T., et al. 1999. Mol Cell Biol. 19: 8581-90. PMID: 10567582
3. A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP.  |  Pagan, JK., et al. 2007. J Biol Chem. 282: 15248-57. PMID: 17379597
4. CtBP- an emerging oncogene and novel small molecule drug target: Advances in the understanding of its oncogenic action and identification of therapeutic inhibitors.  |  Dcona, MM., et al. 2017. Cancer Biol Ther. 18: 379-391. PMID: 28532298
5. Inhibition of the CtBP complex and FBXO11 enhances MHC class II expression and anti-cancer immune responses.  |  Chan, KL., et al. 2022. Cancer Cell. 40: 1190-1206.e9. PMID: 36179686
6. Metabolic modulation of CtBP dimeric status impacts the repression of DNA damage repair genes and the platinum sensitivity of ovarian cancer.  |  Li, J., et al. 2023. Int J Biol Sci. 19: 2081-2096. PMID: 37151877
7. The CtBP binding domain in the adenovirus E1A protein controls CR1-dependent transactivation.  |  Sollerbrant, K., et al. 1996. Nucleic Acids Res. 24: 2578-84. PMID: 8692699
8. Interaction between a cellular protein that binds to the C-terminal region of adenovirus E1A (CtBP) and a novel cellular protein is disrupted by E1A through a conserved PLDLS motif.  |  Schaeper, U., et al. 1998. J Biol Chem. 273: 8549-52. PMID: 9535825
9. Two mechanisms in the action of repressor deltaEF1: binding site competition with an activator and active repression.  |  Sekido, R., et al. 1997. Genes Cells. 2: 771-83. PMID: 9544704
10. Cloning and characterization of mCtBP2, a co-repressor that associates with basic Krüppel-like factor and other mammalian transcriptional regulators.  |  Turner, J. and Crossley, M. 1998. EMBO J. 17: 5129-40. PMID: 9724649
11. The C-terminal (BRCT) domains of BRCA1 interact in vivo with CtIP, a protein implicated in the CtBP pathway of transcriptional repression.  |  Yu, X., et al. 1998. J Biol Chem. 273: 25388-92. PMID: 9738006
12. Characterization of a carboxy-terminal BRCA1 interacting protein.  |  Wong, AK., et al. 1998. Oncogene. 17: 2279-85. PMID: 9811458

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CtBP Antibody (E-12) has 42 citations in a variety of scientific publications.

Primary antibodies like Anti-CtBP Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CtBP Antibody (E-12) Specifications

Alternate Product Name: CtBP

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-440

Epitope Target: CtBP

Epitope Species: human

Product Reactivity/Detection: CtBP1 and CtBP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 42 citations in various scientific publications

Available Conjugates/Forms for CtBP Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin