CtBP Antibody (B-3): sc-55502

CtBP Antibody (B-3) is a mouse monoclonal IgG2a antibody that detects CtBP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CtBP, or C-terminal binding protein, plays a crucial role as a transcriptional corepressor, influencing various cellular processes by modulating gene expression. anti-CtBP antibody (B-3) is particularly important for studying cellular development and differentiation, as CtBP interacts with a range of transcription factors to repress target genes. Notably, CtBP is involved in the regulation of the p21 promoter in response to DNA damage, a critical function that helps maintain genomic stability. The interaction between CtBP and CtIP, a protein that recognizes distinct motifs, is essential for this regulatory mechanism, as CtIP binds to the BRCT repeats of the breast cancer gene BRCA1, thereby enabling CtBP to influence BRCA1 activity. The ability of CtBP to form complexes with other proteins, such as δEF1, further underscores its significance in transcriptional silencing during skeletal and T-cell development. With versatile applications and vital role in cellular regulation, CtBP monoclonal antibody (B-3) serves as an invaluable tool for researchers studying gene expression and its implications in various biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CtBP Antibody (B-3) References:

1. Coordinated histone modifications mediated by a CtBP co-repressor complex.  |  Shi, Y., et al. 2003. Nature. 422: 735-8. PMID: 12700765
2. Corepressor CtBP and nuclear speckle protein Pnn/DRS differentially modulate transcription and splicing of the E-cadherin gene.  |  Alpatov, R., et al. 2008. Mol Cell Biol. 28: 1584-95. PMID: 18086895
3. The oligomeric state of CtBP determines its role as a transcriptional co-activator and co-repressor of Wingless targets.  |  Bhambhani, C., et al. 2011. EMBO J. 30: 2031-43. PMID: 21468031
4. Genome-wide profiles of CtBP link metabolism with genome stability and epithelial reprogramming in breast cancer.  |  Di, LJ., et al. 2013. Nat Commun. 4: 1449. PMID: 23385593
5. CtBP maintains cancer cell growth and metabolic homeostasis via regulating SIRT4.  |  Wang, L., et al. 2015. Cell Death Dis. 6: e1620. PMID: 25633289
6. Structure-guided design of a high affinity inhibitor to human CtBP.  |  Hilbert, BJ., et al. 2015. ACS Chem Biol. 10: 1118-27. PMID: 25636004
7. Assembly of human C-terminal binding protein (CtBP) into tetramers.  |  Bellesis, AG., et al. 2018. J Biol Chem. 293: 9101-9112. PMID: 29700119
8. CtBP-a targetable dependency for tumor-initiating cell activity and metastasis in pancreatic adenocarcinoma.  |  Chawla, AT., et al. 2019. Oncogenesis. 8: 55. PMID: 31586042
9. CtBP determines ovarian cancer cell fate through repression of death receptors.  |  Ding, B., et al. 2020. Cell Death Dis. 11: 286. PMID: 32332713
10. CtBP proteins transactivate matrix metalloproteinases and proinflammatory cytokines to mediate the pathogenesis of abdominal aortic aneurysm.  |  Bai, L., et al. 2022. Exp Cell Res. 421: 113386. PMID: 36244410

The preferred CtBP antibody is CtBP Antibody (E-12): sc-17759.