CstF-64 Antibody (B-3): sc-166647

CstF-64 Antibody (B-3) is a mouse monoclonal IgG1 antibody that detects CstF-64 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CstF-64 plays a crucial role in the polyadenylation of mRNA precursors, a vital process for gene expression regulation, as CstF-64 is involved in the endonucleolytic cleavage of polyadenylation substrates. CstF-64 is essential for the proper maturation of mRNA, influencing the stability and translation efficiency of the transcripts. Notably, CstF-64 is expressed in all somatic cells and is particularly important during B cell activation, where CstF-64 concentration increases to facilitate the switch of immunoglobulin M (IgM) heavy chain mRNA from membrane-bound to secreted forms. This switch is critical for the immune response, as B cells can produce antibodies that effectively neutralize pathogens. The gene encoding CstF-64, designated CSTF2, is located on the X chromosome, highlighting CstF-64′s significance in cellular processes and potential implications in X-linked disorders. Anti-CstF-64 antibody (B-3) is an invaluable tool for researchers studying mRNA processing and its impact on gene expression in various biological contexts.

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CstF-64 Antibody (B-3) References:

1. Elevated levels of the 64-kDa cleavage stimulatory factor (CstF-64) in lipopolysaccharide-stimulated macrophages influence gene expression and induce alternative poly(A) site selection.  |  Shell, SA., et al. 2005. J Biol Chem. 280: 39950-61. PMID: 16207706
2. Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers.  |  Monarez, RR., et al. 2007. Biochem J. 401: 651-8. PMID: 17029590
3. A family of splice variants of CstF-64 expressed in vertebrate nervous systems.  |  Shankarling, GS., et al. 2009. BMC Mol Biol. 10: 22. PMID: 19284619
4. Enterovirus 71 3C protease cleaves a novel target CstF-64 and inhibits cellular polyadenylation.  |  Weng, KF., et al. 2009. PLoS Pathog. 5: e1000593. PMID: 19779565
5. The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.  |  Hockert, JA., et al. 2010. J Biol Chem. 285: 695-704. PMID: 19887456
6. Interactions of CstF-64, CstF-77, and symplekin: implications on localisation and function.  |  Ruepp, MD., et al. 2011. Mol Biol Cell. 22: 91-104. PMID: 21119002
7. CstF64: cell cycle regulation and functional role in 3' end processing of replication-dependent histone mRNAs.  |  Romeo, V., et al. 2014. Mol Cell Biol. 34: 4272-84. PMID: 25266659
8. CstF-64 is necessary for endoderm differentiation resulting in cardiomyocyte defects.  |  Youngblood, BA. and MacDonald, CC. 2014. Stem Cell Res. 13: 413-21. PMID: 25460602
9. The structural basis of CstF-77 modulation of cleavage and polyadenylation through stimulation of CstF-64 activity.  |  Grozdanov, PN., et al. 2018. Nucleic Acids Res. 46: 12022-12039. PMID: 30257008
10. The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation.  |  Takagaki, Y., et al. 1996. Cell. 87: 941-52. PMID: 8945520

The preferred CstF-64 antibody is CstF-64 Antibody (H-1): sc-398862.