CstF-64 Antibody (H-1): sc-398862

CstF-64 Antibody (H-1) is a mouse monoclonal IgM antibody that detects CstF-64 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-CstF-64 antibody (H-1) is available as the non-conjugated form. CstF-64 plays a crucial role in polyadenylation of mRNA precursors, a vital process for gene expression regulation. CstF-64 functions as part of the cleavage stimulation factor (CstF) complex, essential for endonucleolytic cleavage of polyadenylation substrates. An RNA binding domain in CstF-64 facilitates RNA interaction, influencing mRNA stability and translation. CstF-64 expression occurs in all somatic cells and increases during B cell activation to switch IgM heavy chain mRNA expression from membrane-bound to secreted forms. This switch enables B cells to produce antibodies for bloodstream secretion. A larger variant, t CstF-64, expresses in meiotic and postmeiotic cells, demonstrating diverse roles in different cellular contexts. CSTF2, the gene encoding CstF-64, resides on the X chromosome, while an autosomal gene encodes t CstF-64, showing complex regulation and function across various tissues.

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CstF-64 Antibody (H-1) References:

1. Two distinct forms of the 64,000 Mr protein of the cleavage stimulation factor are expressed in mouse male germ cells.  |  Wallace, AM., et al. 1999. Proc Natl Acad Sci U S A. 96: 6763-8. PMID: 10359786
2. Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50.  |  Kleiman, FE. and Manley, JL. 1999. Science. 285: 1576-9. PMID: 10477523
3. The Drosophila homologue of the 64 kDa subunit of cleavage stimulation factor interacts with the 77 kDa subunit encoded by the suppressor of forked gene.  |  Hatton, LS., et al. 2000. Nucleic Acids Res. 28: 520-6. PMID: 10606651
4. Complex protein interactions within the human polyadenylation machinery identify a novel component.  |  Takagaki, Y. and Manley, JL. 2000. Mol Cell Biol. 20: 1515-25. PMID: 10669729
5. The gene for a variant form of the polyadenylation protein CstF-64 is on chromosome 19 and is expressed in pachytene spermatocytes in mice.  |  Dass, B., et al. 2001. J Biol Chem. 276: 8044-50. PMID: 11113135
6. Elevated levels of the polyadenylation factor CstF 64 enhance formation of the 1kB Testis brain RNA-binding protein (TB-RBP) mRNA in male germ cells.  |  Chennathukuzhi, VM., et al. 2001. Mol Reprod Dev. 58: 460-9. PMID: 11241784
7. A multispecies comparison of the metazoan 3'-processing downstream elements and the CstF-64 RNA recognition motif.  |  Salisbury, J., et al. 2006. BMC Genomics. 7: 55. PMID: 16542450
8. A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs.  |  Takagaki, Y., et al. 1990. Genes Dev. 4: 2112-20. PMID: 1980119
9. Transcriptome-wide analyses of CstF64-RNA interactions in global regulation of mRNA alternative polyadenylation.  |  Yao, C., et al. 2012. Proc Natl Acad Sci U S A. 109: 18773-8. PMID: 23112178
10. CstF-64 supports pluripotency and regulates cell cycle progression in embryonic stem cells through histone 3' end processing.  |  Youngblood, BA., et al. 2014. Nucleic Acids Res. 42: 8330-42. PMID: 24957598
11. The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation.  |  Takagaki, Y., et al. 1996. Cell. 87: 941-52. PMID: 8945520
12. Levels of polyadenylation factor CstF-64 control IgM heavy chain mRNA accumulation and other events associated with B cell differentiation.  |  Takagaki, Y. and Manley, JL. 1998. Mol Cell. 2: 761-71. PMID: 9885564

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CstF-64 Antibody (H-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-CstF-64 Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CstF-64 Antibody (H-1) Specifications

Alternate Product Name: CstF-64

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 278-577

Epitope Target: CstF-64

Epitope Species: human

Product Reactivity/Detection: CstF-64 and CstF-64T

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for CstF-64 Antibody (H-1): N/A