Csk Antibody (E-3): sc-166560

Csk Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Csk protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Csk antibody (E-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Csk protein plays a crucial role in regulating cellular signaling pathways by phosphorylating the C-terminal tyrosine residue of Src family kinases, specifically Y527, which is essential for maintaining the inactive state of these kinases. This phosphorylation event prevents aberrant activation of Src kinases, which can lead to uncontrolled cell proliferation and cancer. Csk has been shown to downregulate other Src family members, such as Lyn, Fyn, and Lck, through similar phosphorylation mechanisms, thereby maintaining cellular homeostasis. Csk′s ability to modulate these signaling pathways underscores its importance in various physiological processes and potential as a therapeutic target in diseases characterized by dysregulated Src family kinase activity.

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Csk Antibody (E-3) References:

1. Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src.  |  Nada, S., et al. 1991. Nature. 351: 69-72. PMID: 1709258
2. A protein tyrosine kinase involved in regulation of pp60c-src function.  |  Okada, M. and Nakagawa, H. 1989. J Biol Chem. 264: 20886-93. PMID: 2480346
3. Role of Csk in intestinal epithelial barrier function and protection against colitis.  |  Sun, C., et al. 2018. Biochem Biophys Res Commun. 504: 109-114. PMID: 30173891
4. Crk mediates Csk-Hippo signaling independently of Yap tyrosine phosphorylation to induce cell extrusion.  |  Hannan, A., et al. 2024. bioRxiv.. PMID: 39005335
5. Characterization of the human CSK locus.  |  Bräuninger, A., et al. 1993. Oncogene. 8: 1365-9. PMID: 7683131
6. The when and how of Src regulation.  |  Cooper, JA. and Howell, B. 1993. Cell. 73: 1051-4. PMID: 7685656
7. Disruption of the csk gene, encoding a negative regulator of Src family tyrosine kinases, leads to neural tube defects and embryonic lethality in mice.  |  Imamoto, A. and Soriano, P. 1993. Cell. 73: 1117-24. PMID: 7685657
8. Csk inhibition of c-Src activity requires both the SH2 and SH3 domains of Src.  |  Superti-Furga, G., et al. 1993. EMBO J. 12: 2625-34. PMID: 7687537
9. Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk.  |  Chow, LM., et al. 1993. Nature. 365: 156-60. PMID: 8371758
10. Constitutive activation of Src family kinases in mouse embryos that lack Csk.  |  Nada, S., et al. 1993. Cell. 73: 1125-35. PMID: 8513497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Csk Antibody (E-3) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Csk Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Csk Antibody (E-3) Specifications

Alternate Product Name: C-Terminal Src Kinase

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 431-450

Epitope Target: Csk

Epitope Species: human

Product Reactivity/Detection: Csk p50

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Csk Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin