CRX Antibody (A-9): sc-377138

CRX Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRX (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The cone-rod homeobox-containing gene (CRX) encodes a crucial transcription factor that plays a significant role in the development and function of photoreceptors in the retina, specifically by regulating the expression of key genes such as opsin and rhodopsin. CRX is primarily located in the nuclei of photoreceptor cells, where CRX binds to the OTX motif (TAATCC/A) upstream of photoreceptor genes, thereby influencing their transcription. This localization is vital for proper functioning of the visual system, as disruptions in CRX expression can lead to severe visual pathologies, including cone-rod dystrophy and Leber congenital amaurosis. Additionally, CRX is expressed in the pineal gland, where CRX may regulate circadian rhythms by controlling melatonin synthesis. Mutations in CRX can result in a range of visual impairments, and while all characterized mutations lead to disease in heterozygotes, there is no established correlation between specific mutations and resulting phenotypes. Understanding CRX function and localization is essential for elucidating mechanisms underlying these visual disorders and developing potential therapeutic strategies.

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CRX Antibody (A-9) References:

1. Molecular cloning of the cone-rod homeobox gene (Crx) from the rat and its temporal expression pattern in the retina under a daily light-dark cycle.  |  Sakamoto, K., et al. 1999. Neurosci Lett. 261: 101-4. PMID: 10081937
2. Retinopathy and attenuated circadian entrainment in Crx-deficient mice.  |  Furukawa, T., et al. 1999. Nat Genet. 23: 466-70. PMID: 10581037
3. Transcriptional regulation of the chicken hydroxyindole-O-methyltransferase gene by the cone-rod homeobox-containing protein.  |  Bernard, M., et al. 2001. J Neurochem. 79: 248-57. PMID: 11677252
4. Dominant Leber congenital amaurosis, cone-rod degeneration, and retinitis pigmentosa caused by mutant versions of the transcription factor CRX.  |  Rivolta, C., et al. 2001. Hum Mutat. 18: 488-98. PMID: 11748842
5. Novel frameshift mutations in CRX associated with Leber congenital amaurosis.  |  Rivolta, C., et al. 2001. Hum Mutat. 18: 550-1. PMID: 11748859
6. Otx5 regulates genes that show circadian expression in the zebrafish pineal complex.  |  Gamse, JT., et al. 2002. Nat Genet. 30: 117-21. PMID: 11753388
7. The mouse Crx 5'-upstream transgene sequence directs cell-specific and developmentally regulated expression in retinal photoreceptor cells.  |  Furukawa, A., et al. 2002. J Neurosci. 22: 1640-7. PMID: 11880494
8. The photoreceptor-specific nuclear receptor Nr2e3 interacts with Crx and exerts opposing effects on the transcription of rod versus cone genes.  |  Peng, GH., et al. 2005. Hum Mol Genet. 14: 747-64. PMID: 15689355
9. Hypothalamic neurosecretory and circadian vasopressinergic neuronal systems in the blind cone-rod homeobox knockout mouse (Crx-/-) and the 129sv wild-type mouse.  |  Rovsing, L., et al. 2013. J Comp Neurol. 521: 4061-74. PMID: 23784879
10. RETINA-specific expression of Kcnv2 is controlled by cone-rod homeobox (Crx) and neural retina leucine zipper (Nrl).  |  Aslanidis, A., et al. 2014. Adv Exp Med Biol. 801: 31-41. PMID: 24664678
11. Association of CRX genotypes and retinal phenotypes confounded by variable expressivity and electronegative electroretinogram.  |  Nishiguchi, KM., et al. 2020. Clin Exp Ophthalmol. 48: 644-657. PMID: 32112665
12. Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation.  |  Furukawa, T., et al. 1997. Cell. 91: 531-41. PMID: 9390562

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRX Antibody (A-9) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-CRX Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRX Antibody (A-9) Specifications

Alternate Product Name: CRX

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 111-143

Epitope Target: CRX

Epitope Species: human

Product Reactivity/Detection: CRX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for CRX Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent