CRTAM Antibody (C-12): sc-390581
- CRTAM Antibody (C-12) is a mouse monoclonal IgG1 κ CRTAM antibody provided at 200 µg/ml
- raised against amino acids 287-342 mapping near the C-terminus of CRTAM of mouse origin
- CRTAM Antibody (C-12) is recommended for detection of CRTAM of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CRTAM Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CRTAM Antibody (C-12) for WB applications. These reagents are now offered in bundles with CRTAM Antibody (C-12) (see ordering information below).
QUICK LINKS
SEE ALSO...
CRTAM Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRTAM protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRTAM (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Class-I MHC-restricted T cell associated molecule (CRTAM) serves as a crucial receptor predominantly expressed on activated cytotoxic lymphocytes, playing a significant role in their adhesion, interaction, and migration. CRTAM is one of the most highly expressed surface markers on activated human natural killer T cells and CD8 T cells, which underscores CRTAM′s potential as a diagnostic tool in various human viral and autoimmune diseases. CRTAM interacts with nectin-like protein 2 (Necl2), a protein essential for regulating interleukin-22 expression, thereby influencing immune responses. The Necl2/CRTAM interaction mediates cell-to-cell interactions, which may extend to neuronal interactions, highlighting CRTAM′s importance in both immune and nervous system functions. CRTAM expression in Purkinje neurons within the cerebellum suggests involvement in neural signaling pathways and potential implications in neurobiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CRTAM Antibody (C-12) References:
- A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM). | Kennedy, J., et al. 2000. J Leukoc Biol. 67: 725-34. PMID: 10811014
- Implications of nectin-like molecule-2/IGSF4/RA175/SgIGSF/TSLC1/SynCAM1 in cell-cell adhesion and transmembrane protein localization in epithelial cells. | Shingai, T., et al. 2003. J Biol Chem. 278: 35421-7. PMID: 12826663
- Heparin and modified heparin inhibit complement activation in vivo. | Weiler, JM., et al. 1992. J Immunol. 148: 3210-5. PMID: 1578145
- The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM. | Boles, KS., et al. 2005. Blood. 106: 779-86. PMID: 15811952
- Heterotypic interaction of CRTAM with Necl2 induces cell adhesion on activated NK cells and CD8+ T cells. | Arase, N., et al. 2005. Int Immunol. 17: 1227-37. PMID: 16091383
- Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes. | Patiño-Lopez, G., et al. 2006. J Neuroimmunol. 171: 145-55. PMID: 16300832
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRTAM Antibody (C-12) | sc-390581 | 200 µg/ml | $316.00 | |||
CRTAM Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-530195 | 200 µg Ab; 10 µg BP | $354.00 | |||
CRTAM Antibody (C-12): m-IgGκ BP-HRP Bundle | sc-523589 | 200 µg Ab, 40 µg BP | $354.00 | |||
CRTAM Antibody (C-12): m-IgG1 BP-HRP Bundle | sc-543921 | 200 µg Ab; 20 µg BP | $354.00 | |||
CRTAM Antibody (C-12) AC | sc-390581 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CRTAM Antibody (C-12) HRP | sc-390581 HRP | 200 µg/ml | $316.00 | |||
CRTAM Antibody (C-12) FITC | sc-390581 FITC | 200 µg/ml | $330.00 | |||
CRTAM Antibody (C-12) PE | sc-390581 PE | 200 µg/ml | $343.00 | |||
CRTAM Antibody (C-12) Alexa Fluor® 488 | sc-390581 AF488 | 200 µg/ml | $357.00 | |||
CRTAM Antibody (C-12) Alexa Fluor® 546 | sc-390581 AF546 | 200 µg/ml | $357.00 | |||
CRTAM Antibody (C-12) Alexa Fluor® 594 | sc-390581 AF594 | 200 µg/ml | $357.00 | |||
CRTAM Antibody (C-12) Alexa Fluor® 647 | sc-390581 AF647 | 200 µg/ml | $357.00 | |||
CRTAM Antibody (C-12) Alexa Fluor® 680 | sc-390581 AF680 | 200 µg/ml | $357.00 | |||
CRTAM Antibody (C-12) Alexa Fluor® 790 | sc-390581 AF790 | 200 µg/ml | $357.00 |