Crk-L Antibody (A-1): sc-365471

Crk-L Antibody (A-1) is a mouse monoclonal IgG2b antibody that detects Crk-L in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Crk-L is a pivotal protein in cellular signaling, playing a crucial role in regulating cell proliferation, differentiation, and survival. Crk-L (A-1) antibody helps researchers study pathways that mediate responses to growth factors and cytokines, which are essential for normal cellular function and development. Crk-L includes one SH2 domain and two SH3 domains, allowing interaction with various proteins to facilitate signal transduction. These domains enable anti-Crk-L antibody (A-1) to detect proteins that bind to phosphorylated tyrosine residues, acting as a scaffold that brings together different signaling molecules for signal propagation within cells. Crk-L monoclonal antibody (A-1) supports research into the gene located on chromosome 22, near the breakpoint region associated with chronic myelogenous leukemia, making this antibody valuable for cancer research and studying signaling networks that govern cellular behavior.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Crk-L Antibody (A-1) References:

1. The proto-oncogene c-Cbl is a positive regulator of Met-induced MAP kinase activation: a role for the adaptor protein Crk.  |  Garcia-Guzman, M., et al. 2000. Oncogene. 19: 4058-65. PMID: 10962563
2. T cell regulation of p62(dok) (Dok1) association with Crk-L.  |  Martelli, MP., et al. 2001. J Biol Chem. 276: 45654-61. PMID: 11553620
3. Adaptor protein Crk is required for ephrin-B1-induced membrane ruffling and focal complex assembly of human aortic endothelial cells.  |  Nagashima, K., et al. 2002. Mol Biol Cell. 13: 4231-42. PMID: 12475948
4. Negative regulation of T cell antigen receptor-mediated Crk-L-C3G signaling and cell adhesion by Cbl-b.  |  Zhang, W., et al. 2003. J Biol Chem. 278: 23978-83. PMID: 12697763
5. Dok-7 regulates neuromuscular synapse formation by recruiting Crk and Crk-L.  |  Hallock, PT., et al. 2010. Genes Dev. 24: 2451-61. PMID: 21041412
6. The adaptor protein Crk controls activation and inhibition of natural killer cells.  |  Liu, D., et al. 2012. Immunity. 36: 600-11. PMID: 22464172
7. Adaptor protein CRK induces epithelial-mesenchymal transition and metastasis of bladder cancer cells through HGF/c-Met feedback loop.  |  Matsumoto, R., et al. 2015. Cancer Sci. 106: 709-717. PMID: 25816892
8. Reorganization of the host cell Crk(L)-PI3 kinase signaling complex by the influenza A virus NS1 protein.  |  Ylösmäki, L., et al. 2015. Virology. 484: 146-152. PMID: 26099693
9. Sos, Vav, and C3G participate in B cell receptor-induced signaling pathways and differentially associate with Shc-Grb2, Crk, and Crk-L adaptors.  |  Smit, L., et al. 1996. J Biol Chem. 271: 8564-9. PMID: 8621483
10. Activation of nuclear factor of activated T cells-(NFAT) and activating protein 1 (AP-1) by oncogenic 70Z Cbl requires an intact phosphotyrosine binding domain but not Crk(L) or p85 phosphatidylinositol 3-kinase association.  |  van Leeuwen, JE., et al. 1999. J Biol Chem. 274: 5153-62. PMID: 9988765

The preferred Crk-L antibody is Crk-L Antibody (B-1): sc-365092.