Crk-L Antibody (B-1) recommended for detection of Crk-L of mouse, rat and human origin by WB, IP, IF and ELISA

Crk-L Antibody (B-1): sc-365092

Crk-L Antibody (B-1) is rated 4.8 out of 5 by 4.
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Datasheets

    • Anti-Crk-L Antibody (B-1) is a mouse monoclonal IgG1 (kappa light chain) Crk-L antibody provided at 200 µg/ml
    • raised against amino acids 181-242 of Crk-L of human origin
    • Anti-Crk-L Antibody (B-1) is recommended for detection of Crk-L of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Crk-L Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Crk-L (B-1): sc-365092.

1) What is the cost of the AF647-conjugated antibody "sc-365092 AF647"? 2) Will this Crk-L antibody also stain Crk II?

Asked by: jmjerome
Thank you for your question. We are in the process of updating the pricing and ordering information for our conjugates on the website and this information should be available soon. The cost for sc-365092 AF647 is $314 for 200 µg in 1 ml. This monoclonal antibody is specific for Crk-L and is not expected to cross-react with Crk II.
Answered by: Technical Support
Date published: 2017-12-02

I want to know if the sc-365092: Crk-L (B-1) mouse monoclonal antibody can detect the phosphorylated Crkl? I know that anti-CRKL antibody (C-20, Santa Cruz) can detects the phosphorylated and unphosphorylated protein fractions,but it was off market.

Asked by: yanhua
Thank you for your question. Yes, this antibody would be able to detect both phosphorylated and non-phosphorylated Crk-L. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-10-23

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-365092: Crk-L (B-1) mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Good for WB Works well in Western Blot with a variety of cell lysates at a 1:100 dilution with 50 ug of protein.
Date published: 2017-02-06
Rated 4 out of 5 by from Very good antibody for Western blotting This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a relatively potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It clearly detected an appropriately sized ~36 kDa target protein in these immunoblots, which is only slightly higher than the predicted molecular mass of 33.8 kDa for Crk-L.
Date published: 2016-10-13
Rated 5 out of 5 by from Nice Western Blot data of Crk Nice Western Blot data of Crk-L expression in HeLa, A-431 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Great Western Blot data of Crk Great Western Blot data of Crk-L expression in non-transfected 293T, human Crk-L transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2014-01-06
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