Crk-L Antibody (B-1): sc-365092

Crk-L Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Crk-L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Crk-L (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Crk-L protein plays a crucial role in cellular signaling pathways, particularly in regulating cell growth, differentiation, and survival, making Crk-L an important target for understanding oncogenic processes. Located in the cytoplasm, Crk-L interacts with various signaling molecules, facilitating signal transmission from cell surface receptors to downstream effectors, which is vital for maintaining normal cellular functions and responding to external stimuli. Crk-L protein is characterized by one SH2 domain and two SH3 domains, allowing binding to phosphorylated tyrosine residues and mediating protein-protein interactions. This structural configuration is essential for signal transduction, as Crk-L functions as an adaptor protein linking different signaling pathways, thereby influencing cellular responses to growth factors and other stimuli. The gene encoding Crk-L is located on chromosome 22, band 11, near the chronic myelogenous leukemia breakpoint region, highlighting Crk-L′s potential relevance in cancer research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Crk-L Antibody (B-1) References:

1. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.  |  Lowenstein, EJ., et al. 1992. Cell. 70: 431-42. PMID: 1322798
2. Phosphorylation of Nck in response to a variety of receptors, phorbol myristate acetate, and cyclic AMP.  |  Park, D. and Rhee, SG. 1992. Mol Cell Biol. 12: 5816-23. PMID: 1333046
3. SH2 and SH3 domains: from structure to function.  |  Pawson, T. and Gish, GD. 1992. Cell. 71: 359-62. PMID: 1423600
4. Tyrosine-phosphorylated SOCS3 interacts with the Nck and Crk-L adapter proteins and regulates Nck activation.  |  Sitko, JC., et al. 2004. J Biol Chem. 279: 37662-9. PMID: 15173187
5. Missing links between receptors and Ras.  |  Williams, LT. 1992. Curr Biol. 2: 601-3. PMID: 15336035
6. Activation of Epstein-Barr virus/C3d receptor (gp140, CR2, CD21) on human B lymphoma cell surface triggers Cbl tyrosine phosphorylation, its association with p85 subunit, Crk-L and Syk and its dissociation with Vav.  |  Lottin-Divoux, S., et al. 2006. Cell Signal. 18: 1219-25. PMID: 16289966
7. Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L.  |  Gangenahalli, GU., et al. 2005. Stem Cells Dev. 14: 470-7. PMID: 16305332
8. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.  |  Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
9. Nck, a melanoma cDNA encoding a cytoplasmic protein consisting of the src homology units SH2 and SH3.  |  Lehmann, JM., et al. 1990. Nucleic Acids Res. 18: 1048. PMID: 2107526
10. The adaptor protein Crk in immune response.  |  Liu, D. 2014. Immunol Cell Biol. 92: 80-9. PMID: 24165979
11. A novel viral oncogene with structural similarity to phospholipase C.  |  Mayer, BJ., et al. 1988. Nature. 332: 272-5. PMID: 2450282
12. Isolation and chromosomal localization of CRKL, a human crk-like gene.  |  ten Hoeve, J., et al. 1993. Oncogene. 8: 2469-74. PMID: 8361759

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Crk-L Antibody (B-1) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Crk-L Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Crk-L Antibody (B-1) Specifications

Alternate Product Name: CRKL

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 181-242

Epitope Target: Crk-L

Epitope Species: human

Product Reactivity/Detection: Crk-L

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Crk-L Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin