Crk I/II Antibody (D-6): sc-393160

Crk I/II Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Crk I/II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Crk I/II antibody (D-6) is available as a non-conjugated format. The Crk family of adapter proteins, including Crk-I, Crk-II, and Crk-L, features SH2 and SH3 domains crucial for cellular signaling. These domains facilitate interactions with phosphotyrosine residues and proline-rich motifs, participating in various signaling pathways that regulate cell growth, differentiation, and survival. Crk I and Crk II are products of the same gene through alternative splicing, sharing structural similarities while exhibiting distinct biological functions - Crk-I shows higher transforming activity compared to Crk-II. Crk-II rapidly phosphorylates in response to insulin-like growth factor I (IGF-I), suggesting involvement in IGF-I receptor signaling pathway, critical for cellular responses to growth factors. Crk I and II genes reside on human chromosome 17p13, a region frequently showing deletions and loss of heterozygosity in various cancers, highlighting their potential significance in tumorigenesis and cancer progression.

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Crk I/II Antibody (D-6) References:

1. Mutant mice lacking Crk-II caused by the gene trap insertional mutagenesis: Crk-II is not essential for embryonic development.  |  Imaizumi, T., et al. 1999. Biochem Biophys Res Commun. 266: 569-74. PMID: 10600543
2. Phosphorylation of c-Crk II on the negative regulatory Tyr222 mediates nerve growth factor-induced cell spreading and morphogenesis.  |  Escalante, M., et al. 2000. J Biol Chem. 275: 24787-97. PMID: 10825157
3. Activation of the focal adhesion kinase signaling pathway by structural alterations in the carboxyl-terminal region of c-Crk II.  |  Zvara, A., et al. 2001. Oncogene. 20: 951-61. PMID: 11314030
4. The insulin-like growth factor I receptor-induced interaction of insulin receptor substrate-4 and Crk-II.  |  Karas, M., et al. 2001. Endocrinology. 142: 1835-40. PMID: 11316748
5. A Crk-II/TC10 signaling pathway is required for osmotic shock-stimulated glucose transport.  |  Gual, P., et al. 2002. J Biol Chem. 277: 43980-6. PMID: 12215429
6. Transactivation of Abl by the Crk II adapter protein requires a PNAY sequence in the Crk C-terminal SH3 domain.  |  Reichman, C., et al. 2005. Oncogene. 24: 8187-99. PMID: 16158059
7. Two species of human CRK cDNA encode proteins with distinct biological activities.  |  Matsuda, M., et al. 1992. Mol Cell Biol. 12: 3482-9. PMID: 1630456
8. Proapoptotic function of the nuclear Crk II adaptor protein.  |  Kar, B., et al. 2007. Biochemistry. 46: 10828-40. PMID: 17764157
9. NDRG1 and CRK-I/II are regulators of endothelial cell migration under Intermittent Hypoxia.  |  Toffoli, S., et al. 2009. Angiogenesis. 12: 339-54. PMID: 19760510
10. Crk II silencing down-regulates IGF-IR and inhibits migration and invasion of prostate cancer cells.  |  Dhupkar, P., et al. 2016. Biochem Biophys Rep. 8: 382-388. PMID: 28955980
11. CRK proto-oncogene maps to human chromosome band 17p13.  |  Fioretos, T., et al. 1993. Oncogene. 8: 2853-5. PMID: 8378094
12. Interaction in vitro of the product of the c-Crk-II proto-oncogene with the insulin-like growth factor I receptor.  |  Koval, AP., et al. 1998. Biochem J. 330 (Pt 2): 923-32. PMID: 9480911

The preferred Crk II antibody is Crk II Antibody (B-4): sc-390132.