CRIF1 Antibody (H-9): sc-374122

CRIF1 Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRIF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRIF1 (H-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CRIF1 protein, also known as GADD45GIP1, PLINP1, PRG6, or CKBBP2, is a 222 amino acid nuclear protein that plays a crucial role in regulating apoptosis and cell cycle progression. CRIF1 is expressed in a variety of tissues, including heart, thyroid, trachea, kidney, ovary, pancreas, testis, and stomach, where CRIF1 functions as a negative regulator of G1 to S phase transition in cell cycle. CRIF1 achieves this by interacting with GADD45 proteins to inhibit cyclin-dependent kinases (Cdks) activity, thereby preventing premature cell cycle progression. Notably, phosphorylation of CRIF1 by casein kinase II is a significant post-translational modification that reduces CRIF1 activity, promoting cellular proliferation. Balance of CRIF1 expression is critical, as overexpression can lead to cell cycle arrest at G1 phase, while downregulation by p53 in apoptotic cells can facilitate cell cycle progression, potentially influencing tumor growth and metastasis. Human CRIF1 shares 90% homology with mouse counterpart, indicating a conserved functional role across species, making anti-CRIF1 antibody (H-9) an essential tool for studying cell cycle regulation and apoptosis in various biological contexts.

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CRIF1 Antibody (H-9) References:

1. Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene.  |  Horikoshi, N., et al. 1999. Biochem Biophys Res Commun. 261: 864-9. PMID: 10441517
2. CR6-interacting factor 1 interacts with Gadd45 family proteins and modulates the cell cycle.  |  Chung, HK., et al. 2003. J Biol Chem. 278: 28079-88. PMID: 12716909
3. CR6-interacting factor 1 interacts with orphan nuclear receptor Nur77 and inhibits its transactivation.  |  Park, KC., et al. 2005. Mol Endocrinol. 19: 12-24. PMID: 15459248
4. Phosphorylation of CKBBP2/CRIF1 by protein kinase CKII promotes cell proliferation.  |  Oh, NS., et al. 2007. Gene. 386: 147-53. PMID: 17069992
5. NAC-1 controls cell growth and survival by repressing transcription of Gadd45GIP1, a candidate tumor suppressor.  |  Nakayama, K., et al. 2007. Cancer Res. 67: 8058-64. PMID: 17804717
6. CR6-interacting factor 1 represses the transactivation of androgen receptor by direct interaction.  |  Suh, JH., et al. 2008. Mol Endocrinol. 22: 33-46. PMID: 17885209
7. CRIF1 deficiency suppresses endothelial cell migration via upregulation of RhoGDI2.  |  Nagar, H., et al. 2021. PLoS One. 16: e0256646. PMID: 34437633
8. Multifunctions of CRIF1 in cancers and mitochondrial dysfunction.  |  Jiang, Y., et al. 2022. Front Oncol. 12: 1009948. PMID: 36263222
9. CRIF1 siRNA-Encapsulated PLGA Nanoparticles Suppress Tumor Growth in MCF-7 Human Breast Cancer Cells.  |  Piao, S., et al. 2023. Int J Mol Sci. 24: PMID: 37108616
10. Crif1 deficiency in dopamine neurons triggers early-onset parkinsonism.  |  Heo, JY., et al. 2023. Mol Psychiatry. 28: 4474-4484. PMID: 37648779
11. CRIF1 attenuates doxorubicin-mediated mitochondrial dysfunction and myocardial senescence via regulating PXDN.  |  Zhou, L., et al. 2024. Aging (Albany NY). 16: 5567-5580. PMID: 38517371
12. CRIF1 deficiency induces FOXP3LOW inflammatory non-suppressive regulatory T cells, thereby promoting antitumor immunity.  |  Lee, S., et al. 2024. Sci Adv. 10: eadj9600. PMID: 38536932

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRIF1 Antibody (H-9) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-CRIF1 Antibody (H-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRIF1 Antibody (H-9) Specifications

Alternate Product Name: CRIF1

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-222

Epitope Target: CRIF1

Epitope Species: mouse

Product Reactivity/Detection: CRIF1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for CRIF1 Antibody (H-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin