CREB1 Antibody (D-4): sc-374227
- CREB1 Antibody (D-4) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-341 of CREB-1 of human origin
- recommended for detection of CREB-1A, CREB-1B, CREM and ATF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- TransCruz reagent for ChIP application (sc-374227 X, 200 µg/0.1 ml)
- See CREB1 (D-12): sc-377154 for CREB1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CREB1 Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with CREB1 Antibody (D-4) (see ordering information below).
CREB-1 Antibody (D-4) is a mouse monoclonal IgG2a kappa light chain antibody designed to target amino acids 1-341 of human CREB-1, facilitating the detection of CREB-1A, CREB-1B, CREM, and ATF-1 isoforms across mouse, rat, and human species using western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. CREB-1, also referred to as CREB, CREB1, and cAMP response element-binding protein 1, is a vital transcription factor localized primarily in the cell nucleus, where CREB-1 binds to cAMP response elements (CRE) in the promoters of target genes, thereby regulating essential cellular processes such as metabolism, neuronal plasticity, and cell survival. A key aspect of CREB-1′s functionality involves post-translational modifications, particularly phosphorylation at Serine 133 by protein kinase A, which is crucial for activation and subsequent interaction with the CREB-binding protein (CBP). This phosphorylation event induces a conformational change in CREB-1, enabling the recruitment of CBP and the assembly of the basal transcription complex, thereby initiating the transcription of genes involved in cellular responses to hormonal and stress signals. Additionally, CREB-1 can undergo other post-translational modifications such as acetylation and ubiquitination, which regulate stability, DNA-binding affinity, and interaction with other coactivators, further fine-tuning gene expression. These modifications are essential for the precise control of CREB-1 activity, influencing processes like neoglucogenesis through interactions with the nuclear coactivator PGC-1 and playing significant roles in the pathogenesis of type II diabetes and dilated cardiomyopathy. anti-CREB-1 antibody (D-4) is an indispensable reagent for researchers exploring the complex regulatory mechanisms governed by CREB-1 and its impact on cellular function and disease progression.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CREB1 Antibody (D-4) | sc-374227 | 200 µg/ml | $316.00 | |||
CREB1 Antibody (D-4): m-IgG Fc BP-HRP Bundle | sc-537939 | 200 µg Ab; 10 µg BP | $354.00 | |||
CREB1 Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-535424 | 200 µg Ab; 40 µg BP | $354.00 | |||
CREB1 Antibody (D-4): m-IgG2a BP-HRP Bundle | sc-548153 | 200 µg Ab; 10 µg BP | $354.00 | |||
CREB1 Antibody (D-4) X | sc-374227 X | 200 µg/0.1 ml | $316.00 |