creatine kinase-M Antibody (MM-2): sc-69848

Creatine kinase-M Antibody (MM-2) is a mouse monoclonal IgG1 antibody that detects creatine kinase-M in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Creatine kinase-M is a muscle tissue-specific isoform of creatine kinase, consisting of 381 amino acids, and plays a crucial role in energy metabolism by catalyzing the transfer of phosphate from creatine to adenosine diphosphate (ADP) to regenerate adenosine triphosphate (ATP), the primary energy currency of the cell. This function is particularly important in muscle tissues, where rapid ATP regeneration is essential during periods of high energy demand, such as during intense physical activity. Creatine kinase-M is predominantly found in skeletal muscle and cardiac tissue, where creatine kinase-M helps maintain energy homeostasis and supports muscle contraction. Creatine kinase-M is also involved in the regulation of calcium homeostasis, which is vital for muscle function and overall cellular health. Anti-creatine kinase-M antibody (MM-2) enables researchers to effectively study the expression and regulation of creatine kinase-M in various physiological and pathological contexts, enhancing our understanding of muscle metabolism and related disorders.

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creatine kinase-M Antibody (MM-2) References:

1. Assessment of a creatine kinase isoform M defect as a cause of myotonic dystrophy and the characterization of two novel CKMM polymorphisms.  |  Bailly, J., et al. 1991. Hum Genet. 86: 457-62. PMID: 2016086
2. Tight linkage of creatine kinase (CKMM) to myotonic dystrophy on chromosome 19.  |  Yamaoka, LH., et al. 1990. Neurology. 40: 222-6. PMID: 2300239
3. Spermatogenesis-related change in the synthesis of the creatine kinase B-type and M-type isoforms in human spermatozoa.  |  Huszar, G. and Vigue, L. 1990. Mol Reprod Dev. 25: 258-62. PMID: 2331374
4. Appearance of M-band attached MM-creatine kinase in differentiating chicken heart cells after injection of M-type isoprotein or poly (A+)-RNA enriched for M-type creatine kinase message.  |  Schäfer, B., et al. 1985. Basic Res Cardiol. 80 Suppl 2: 129-33. PMID: 2415105
5. Molecular Characterization and Expression Analysis of Creatine Kinase Muscle (CK-M) Gene in Horse.  |  Do, KT., et al. 2015. Asian-Australas J Anim Sci. 28: 1680-5. PMID: 26580434
6. Myotonic dystrophy is closely linked to the gene for muscle-type creatine kinase (CKMM).  |  Brunner, HG., et al. 1989. Hum Genet. 81: 308-10. PMID: 2703233
7. NcoI RFLP at the creatine kinase-muscle type gene locus (CKMM, chromosome 19).  |  Coerwinkel-Driessen, M., et al. 1988. Nucleic Acids Res. 16: 8743. PMID: 2901730
8. Creatine kinase muscle type specifically interacts with saturated fatty acid- and/or monounsaturated fatty acid-containing phosphatidic acids.  |  Hoshino, F., et al. 2019. Biochem Biophys Res Commun. 513: 1035-1040. PMID: 31010675
9. The Best of Both Worlds: Eliminating Creatine Kinase-Muscle/Brain (CK-MB) Testing in the Emergency Department Leads to Lower Costs Without Missed Clinical Diagnoses.  |  Sahadeo, PA., et al. 2021. Cureus. 13: e15150. PMID: 34164248
10. Expression of creatine kinase M and B mRNAs in treadmill trained rat skeletal muscle.  |  Apple, FS. and Billadello, JJ. 1994. Life Sci. 55: 585-92. PMID: 8046994

The preferred creatine kinase-M antibody is creatine kinase-M Antibody (G-9): sc-365046.