creatine kinase-M Antibody (G-9): sc-365046

Creatine kinase-M Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects creatine kinase-M protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-creatine kinase-M antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Creatine kinase-M plays a crucial role in cellular energy metabolism, particularly in muscle tissues, where creatine kinase-M facilitates rapid regeneration of adenosine triphosphate (ATP) during periods of high energy demand. Creatine kinase-M ensures energy availability for muscle contraction and relaxation processes. Creatine kinase-M maintains cellular energy homeostasis across various tissues, including brain and heart, highlighting significance in both health and disease states. Creatine kinase-M interacts with myofibrillar proteins and various signaling molecules, underscoring importance in muscle physiology and pathology. Creatine kinase-M monoclonal antibody (G-9) enables researchers to gain insights into energy metabolism dynamics in different tissues, contributing to better understanding of muscle-related disorders and potential therapeutic targets.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

creatine kinase-M Antibody (G-9) References:

1. Assessment of a creatine kinase isoform M defect as a cause of myotonic dystrophy and the characterization of two novel CKMM polymorphisms.  |  Bailly, J., et al. 1991. Hum Genet. 86: 457-62. PMID: 2016086
2. Prognostic value of troponin and creatine kinase muscle and brain isoenzyme measurement after noncardiac surgery: a systematic review and meta-analysis.  |  Levy, M., et al. 2011. Anesthesiology. 114: 796-806. PMID: 21336095
3. CK-MM interference with an immunoenzymometric method for quantification of CK-MB in serum.  |  Panteghini, M., et al. 1989. Clin Chem. 35: 901-2. PMID: 2656004
4. Complete nucleotide sequence of the human creatine kinase B gene.  |  Mariman, EC., et al. 1989. Nucleic Acids Res. 17: 6385. PMID: 2771648
5. NcoI RFLP at the creatine kinase-muscle type gene locus (CKMM, chromosome 19).  |  Coerwinkel-Driessen, M., et al. 1988. Nucleic Acids Res. 16: 8743. PMID: 2901730
6. Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase.  |  Haas, RC., et al. 1989. J Biol Chem. 264: 2890-7. PMID: 2914937
7. Two commercial test kits for CK-MM isoforms evaluated for early recognition of acute myocardial infarction.  |  Shah, VD., et al. 1989. Clin Chem. 35: 493-4. PMID: 2920421
8. cDNA cloning and mapping of the human creatine kinase M gene to 19q13.  |  Nigro, JM., et al. 1987. Am J Hum Genet. 40: 115-25. PMID: 3031982
9. Creatine kinase muscle type specifically interacts with saturated fatty acid- and/or monounsaturated fatty acid-containing phosphatidic acids.  |  Hoshino, F., et al. 2019. Biochem Biophys Res Commun. 513: 1035-1040. PMID: 31010675
10. Structure and expression of the human creatine kinase B gene.  |  Mariman, EC., et al. 1987. Genomics. 1: 126-37. PMID: 3692484
11. Creatine kinase in non-muscle tissues and cells.  |  Wallimann, T. and Hemmer, W. 1994. Mol Cell Biochem. 133-134: 193-220. PMID: 7808454
12. Some new aspects of creatine kinase (CK): compartmentation, structure, function and regulation for cellular and mitochondrial bioenergetics and physiology.  |  Wallimann, T., et al. 1998. Biofactors. 8: 229-34. PMID: 9914824

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-creatine kinase-M Antibody (G-9) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-creatine kinase-M Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

creatine kinase-M Antibody (G-9) Specifications

Alternate Product Name: creatine kinase-M

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 9-27

Epitope Target: creatine kinase-M

Epitope Species: human

Product Reactivity/Detection: creatine kinase-M chain

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for creatine kinase-M Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin