creatine kinase-M Antibody (C-4): sc-390053

Creatine kinase-M Antibody (C-4) is a mouse monoclonal IgG1 antibody that detects creatine kinase-M in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Creatine kinase-M is a muscle tissue-specific isoform of creatine kinase, consisting of 381 amino acids, and plays a crucial role in energy metabolism by catalyzing the reversible transfer of phosphate from creatine to adenosine diphosphate (ADP), thereby regenerating adenosine triphosphate (ATP) during periods of high energy demand. This function is particularly important in muscle cells, where ATP is essential for muscle contraction and overall cellular function. The regulation of ATP levels by creatine kinase-M is vital for maintaining energy homeostasis, especially during intense physical activity or stress, as ATP remains readily available for muscle contraction and other metabolic processes. Creatine kinase-M also regulates calcium pumps, which are critical for muscle relaxation and contraction, highlighting significance in both muscle physiology and pathophysiology. Anti-creatine kinase-M antibody (C-4) serves as an invaluable tool for researchers studying muscle metabolism, energy regulation, and related disorders, providing insights into molecular mechanisms underlying muscle function and disease.

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creatine kinase-M Antibody (C-4) References:

1. Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity. Activity kinetics and diagnostic significance in myocardial infarction.  |  Neumeier, D., et al. 1976. Clin Chim Acta. 73: 445-51. PMID: 1000863
2. Putative creatine kinase M-isoform in human sperm is identifiedas the 70-kilodalton heat shock protein HspA2.  |  Huszar, G., et al. 2000. Biol Reprod. 63: 925-32. PMID: 10952940
3. Prospective analysis of creatine kinase muscle-brain fraction and comparison with troponin T to predict cardiac risk and benefit of an invasive strategy in patients with non-ST-elevation acute coronary syndromes.  |  Kleiman, NS., et al. 2002. J Am Coll Cardiol. 40: 1044-50. PMID: 12354426
4. cDNA cloning and mapping of the human creatine kinase M gene to 19q13.  |  Nigro, JM., et al. 1987. Am J Hum Genet. 40: 115-25. PMID: 3031982
5. Levels of C-reactive protein, creatine kinase-muscle and aldolase A are suitable biomarkers to detect the risk factors for osteoarthritic disorders: A novel diagnostic protocol.  |  Ganguly, A. 2019. Caspian J Intern Med. 10: 25-35. PMID: 30858938
6. Evaluation of the GSP Creatine Kinase-MM Assay and Assessment of CK-MM Stability in Newborn, Patient, and Contrived Dried Blood Spots for Newborn Screening for Duchenne Muscular Dystrophy.  |  Migliore, BA., et al. 2022. Int J Neonatal Screen. 8: PMID: 35225934
7. Indices for the age of the creatine kinase M-chain in the blood.  |  Wevers, RA., et al. 1985. Clin Chim Acta. 148: 197-210. PMID: 4042354
8. Elevated serum creatine kinase (CK-MM) in petrol sniffers using leaded or unleaded fuel.  |  Burns, CB., et al. 1994. J Toxicol Clin Toxicol. 32: 527-39. PMID: 7932912
9. Changes in creatine kinase M localization in acute ischemic myocardial cells. Immunoelectron microscopic studies.  |  Otsu, N., et al. 1993. Circ Res. 73: 935-42. PMID: 8403263
10. Biochemical markers of early and late spermatogenesis: relationship between the lactate dehydrogenase-X and creatine kinase-M isoform concentrations in human spermatozoa.  |  Lalwani, S., et al. 1996. Mol Reprod Dev. 43: 495-502. PMID: 9052941

The preferred creatine kinase-M antibody is creatine kinase-M Antibody (G-9): sc-365046.