CRBP I Antibody (B-8): sc-271208

CRBP I Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRBP I of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CRBP I (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The cellular retinol-binding proteins, including CRBP I, play a crucial role in the transport and metabolism of vitamin A, which is vital for numerous biological processes such as cell growth, differentiation, and vision. CRBP I is primarily located in the cytoplasm and is essential for the cellular uptake of retinol, where CRBP I solubilizes and detoxifies this hydrophobic molecule, facilitating transport and conversion into retinoic acid, the active form of vitamin A. The structure of CRBP I, characterized by a β-barrel formed from ten antiparallel β-strands and two α-helices, provides a protective environment for retinol, ensuring stability and bioavailability for subsequent enzymatic reactions. CRBP I is expressed in various human tissues, including the ovary, adrenal and pituitary glands, and testis, with CRBP I expression being modulated by transforming growth factor beta. Understanding the function and structure of CRBP I is essential for elucidating CRBP I′s role in retinoid metabolism and implications in health and disease.

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CRBP I Antibody (B-8) References:

1. Regulation of vitamin A metabolism-related gene expression.  |  Takase, S., et al. 2000. Br J Nutr. 84 Suppl 2: S217-21. PMID: 11242473
2. Identification, retinoid binding, and x-ray analysis of a human retinol-binding protein.  |  Folli, C., et al. 2001. Proc Natl Acad Sci U S A. 98: 3710-5. PMID: 11274389
3. Regulation of alpha-smooth muscle actin and CRBP-1 expression by retinoic acid and TGF-beta in cultured fibroblasts.  |  Xu, G., et al. 2001. J Cell Physiol. 187: 315-25. PMID: 11319755
4. CRBP suppresses breast cancer cell survival and anchorage-independent growth.  |  Kuppumbatti, YS., et al. 2001. Oncogene. 20: 7413-9. PMID: 11704871
5. DNA methylation of genes linked to retinoid signaling in squamous cell carcinoma of the esophagus: DNA methylation of CRBP1 and TIG1 is associated with tumor stage.  |  Mizuiri, H., et al. 2005. Cancer Sci. 96: 571-7. PMID: 16128742
6. Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer.  |  Yokoi, K., et al. 2017. Br J Cancer. 116: 1046-1056. PMID: 28291773
7. Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1.  |  Silvaroli, JA., et al. 2019. ACS Chem Biol. 14: 434-448. PMID: 30721022
8. Quantitation of cellular retinol-binding protein in human organs.  |  Ong, DE. and Page, DL. 1986. Am J Clin Nutr. 44: 425-30. PMID: 3751963
9. All-trans retinoic acid induces durable tumor immunity in IDH-mutant gliomas by rescuing transcriptional repression of the CRBP1-retinoic acid axis.  |  Rao, A., et al. 2024. bioRxiv.. PMID: 38645178
10. Crystallographic studies on a family of cellular lipophilic transport proteins. Refinement of P2 myelin protein and the structure determination and refinement of cellular retinol-binding protein in complex with all-trans-retinol.  |  Cowan, SW., et al. 1993. J Mol Biol. 230: 1225-46. PMID: 7683727
11. [Cellular retinoid-binding proteins].  |  Okuno, M., et al. 1993. Nihon Rinsho. 51: 879-85. PMID: 8387121
12. Crystal structures of holo and apo-cellular retinol-binding protein II.  |  Winter, NS., et al. 1993. J Mol Biol. 230: 1247-59. PMID: 8487303

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRBP I Antibody (B-8) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-CRBP I Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRBP I Antibody (B-8) Specifications

Alternate Product Name: CRBP I

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-135

Epitope Target: CRBP I

Epitope Species: human

Product Reactivity/Detection: CRBP I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for CRBP I Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin