CPSF3 Antibody (C-3): sc-393001
- CPSF3 Antibody (C-3) is a mouse monoclonal IgG1 κ CPSF3 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 418-562 mapping within an internal region of CPSF3 of human origin
- CPSF3 Antibody (C-3) is recommended for detection of CPSF3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-CPSF3 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CPSF3 Antibody (C-3) for WB applications. These reagents are now offered in bundles with CPSF3 Antibody (C-3) (see ordering information below).
CPSF3 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CPSF3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CPSF3 Antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPSF3 protein plays a crucial role in the processing of the 3′ ends of eukaryotic mRNAs, which is essential for the stability and translation of mRNA. Specifically, CPSF3 is a key component of the cleavage and polyadenylation specificity factor (CPSF) complex, which is responsible for recognizing the AAUAAA signal in pre-mRNA and facilitating the endonucleolytic cleavage and subsequent polyadenylation of the transcript. This process is vital for the maturation of mRNA, as CPSF3 influences mRNA stability, transport, and translation efficiency. The human CPSF3 gene is located on chromosome 2p25.1 and encodes the second shortest subunit of the CPSF complex. Notably, U1 snRNP-A protein (U1A) interacts with CPSF3, enhancing CPSF3 activity by stabilizing the binding of CPSF to AAUAAA-containing RNAs, thereby increasing the efficiency of polyadenylation. This interaction underscores the importance of CPSF3 in gene expression regulation and mRNA processing, making anti-CPSF3 antibody (C-3) an invaluable tool for researchers studying RNA biology and related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CPSF3 Antibody (C-3) References:
- A history of poly A sequences: from formation to factors to function. | Edmonds, M. 2002. Prog Nucleic Acid Res Mol Biol. 71: 285-389. PMID: 12102557
- The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation. | Murthy, KG. and Manley, JL. 1995. Genes Dev. 9: 2672-83. PMID: 7590244
- Interaction between the U1 snRNP-A protein and the 160-kD subunit of cleavage-polyadenylation specificity factor increases polyadenylation efficiency in vitro. | Lutz, CS., et al. 1996. Genes Dev. 10: 325-37. PMID: 8595883
- Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I. | Jenny, A., et al. 1996. Science. 274: 1514-7. PMID: 8929409
- The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. | Barabino, SM., et al. 1997. Genes Dev. 11: 1703-16. PMID: 9224719
- Characterization of a Drosophila homologue of the 160-kDa subunit of the cleavage and polyadenylation specificity factor CPSF. | Salinas, CA., et al. 1998. Mol Gen Genet. 257: 672-80. PMID: 9604891
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPSF3 Antibody (C-3) | sc-393001 | 200 µg/ml | $322.00 | |||
CPSF3 Antibody (C-3): m-IgG Fc BP-HRP Bundle | sc-527659 | 200 µg Ab; 10 µg BP | $361.00 | |||
CPSF3 Antibody (C-3): m-IgG1 BP-HRP Bundle | sc-533032 | 200 µg Ab; 20 µg BP | $361.00 | |||
CPSF3 Antibody (C-3) AC | sc-393001 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CPSF3 Antibody (C-3) HRP | sc-393001 HRP | 200 µg/ml | $322.00 | |||
CPSF3 Antibody (C-3) FITC | sc-393001 FITC | 200 µg/ml | $336.00 | |||
CPSF3 Antibody (C-3) PE | sc-393001 PE | 200 µg/ml | $349.00 | |||
CPSF3 Antibody (C-3) Alexa Fluor® 488 | sc-393001 AF488 | 200 µg/ml | $364.00 | |||
CPSF3 Antibody (C-3) Alexa Fluor® 546 | sc-393001 AF546 | 200 µg/ml | $364.00 | |||
CPSF3 Antibody (C-3) Alexa Fluor® 594 | sc-393001 AF594 | 200 µg/ml | $364.00 | |||
CPSF3 Antibody (C-3) Alexa Fluor® 647 | sc-393001 AF647 | 200 µg/ml | $364.00 | |||
CPSF3 Antibody (C-3) Alexa Fluor® 680 | sc-393001 AF680 | 200 µg/ml | $364.00 | |||
CPSF3 Antibody (C-3) Alexa Fluor® 790 | sc-393001 AF790 | 200 µg/ml | $364.00 |