CPS2 Antibody (F-6): sc-376072
- CPS2 Antibody (F-6) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 1311-1490 mapping within an internal region of CPS2 of human origin
- Anti-CPS2 Antibody (F-6) is recommended for detection of CPS2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CPS2 Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CPS2 Antibody (F-6) for WB and IHC(P) applications. These reagents are now offered in bundles with CPS2 Antibody (F-6) (see ordering information below).
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CPS2 Antibody (F-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects CPS2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CPS2 Antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPS2 plays a crucial role in the multicomplex protein known as carbamoyl-phosphate synthetase-aspartate carbamoyl transferase-dihydro-orotase (CAD), which is essential for pyrimidine biosynthesis. Specifically, CPS2 catalyzes the rate-limiting step in this pathway, making CPS2 vital for the synthesis of nucleotides necessary for DNA and RNA production. Phosphorylation through cyclic AMP-dependent protein kinase regulates CPS2 activity and ensures proper cellular function. CAD is predominantly expressed in the brain and skeletal muscle, highlighting CAD′s importance in these tissues, while carbamoyl phosphate synthetase 1 (CPS1) is found in the liver and is critical for the urea cycle. CPS1 deficiencies can lead to severe metabolic disorders, underscoring CPS2′s significance in maintaining cellular homeostasis and metabolic balance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CPS2 Antibody (F-6) References:
- Functional linkage between the glutaminase and synthetase domains of carbamoyl-phosphate synthetase. Role of serine 44 in carbamoyl-phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (cad). | Hewagama, A., et al. 1999. J Biol Chem. 274: 28240-5. PMID: 10497179
- Regulation of carbamoyl phosphate synthetase by MAP kinase. | Graves, LM., et al. 2000. Nature. 403: 328-32. PMID: 10659854
- Localization of the multifunctional protein CAD in astrocytes of rodent brain. | Cammer, W. and Downing, M. 1991. J Histochem Cytochem. 39: 695-700. PMID: 1673139
- Cloning and sequence of a cDNA encoding human carbamyl phosphate synthetase I: molecular analysis of hyperammonemia. | Haraguchi, Y., et al. 1991. Gene. 107: 335-40. PMID: 1840546
- Phosphorylation and activation of hamster carbamyl phosphate synthetase II by cAMP-dependent protein kinase. A novel mechanism for regulation of pyrimidine nucleotide biosynthesis. | Carrey, EA., et al. 1985. EMBO J. 4: 3735-42. PMID: 4092695
- Phosphorylation and dephosphorylation of carbamoyl-phosphate synthetase II complex of rat ascites hepatoma cells. | Otsuki, T., et al. 1981. J Biochem. 89: 1367-74. PMID: 6115855
- Mice deficient in the urea-cycle enzyme, carbamoyl phosphate synthetase I, die during the early neonatal period from hyperammonemia. | Schofield, JP., et al. 1999. Hepatology. 29: 181-5. PMID: 9862865
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPS2 Antibody (F-6) | sc-376072 | 200 µg/ml | $322.00 | |||
CPS2 Antibody (F-6): m-IgG Fc BP-HRP Bundle | sc-529816 | 200 µg Ab; 10 µg BP | $361.00 | |||
CPS2 Antibody (F-6): m-IgGκ BP-HRP Bundle | sc-522839 | 200 µg Ab, 40 µg BP | $361.00 | |||
CPS2 Antibody (F-6): m-IgG2a BP-HRP Bundle | sc-547384 | 200 µg Ab; 10 µg BP | $361.00 | |||
CPS2 Antibody (F-6) AC | sc-376072 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CPS2 Antibody (F-6) HRP | sc-376072 HRP | 200 µg/ml | $322.00 | |||
CPS2 Antibody (F-6) FITC | sc-376072 FITC | 200 µg/ml | $336.00 | |||
CPS2 Antibody (F-6) PE | sc-376072 PE | 200 µg/ml | $349.00 | |||
CPS2 Antibody (F-6) Alexa Fluor® 488 | sc-376072 AF488 | 200 µg/ml | $364.00 | |||
CPS2 Antibody (F-6) Alexa Fluor® 546 | sc-376072 AF546 | 200 µg/ml | $364.00 | |||
CPS2 Antibody (F-6) Alexa Fluor® 594 | sc-376072 AF594 | 200 µg/ml | $364.00 | |||
CPS2 Antibody (F-6) Alexa Fluor® 647 | sc-376072 AF647 | 200 µg/ml | $364.00 | |||
CPS2 Antibody (F-6) Alexa Fluor® 680 | sc-376072 AF680 | 200 µg/ml | $364.00 | |||
CPS2 Antibody (F-6) Alexa Fluor® 790 | sc-376072 AF790 | 200 µg/ml | $364.00 |