p-CPS2 Antibody (F-4): sc-377559

p-CPS2 Antibody (F-4) is a mouse monoclonal IgM kappa light chain antibody that detects Thr 456 phosphorylated CPS2 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-CPS2 (F-4) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. CPS2 is a crucial component of the multicomplex enzyme carbamoyl-phosphate synthetase-aspartate carbamoyl transferase-dihydro-orotase (CAD), which plays a vital role in pyrimidine biosynthesis by catalyzing the second and third steps of this pathway. Phosphorylation of CPS2 by cyclic AMP-dependent protein kinase enhances activity, facilitating the rate-limiting step in pyrimidine synthesis. This regulation is particularly important as pyrimidines are essential for DNA and RNA synthesis, impacting cell proliferation and metabolism. CAD is predominantly expressed in brain and skeletal muscle tissues, highlighting significance in cellular functions within these organs. A related enzyme, carbamoyl phosphate synthetase 1 (CPS1), is found in the liver and is involved in the urea cycle, underscoring the interconnectedness of metabolic pathways and CPS2′s importance in maintaining cellular homeostasis.

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p-CPS2 Antibody (F-4) References:

1. Functional linkage between the glutaminase and synthetase domains of carbamoyl-phosphate synthetase. Role of serine 44 in carbamoyl-phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (cad).  |  Hewagama, A., et al. 1999. J Biol Chem. 274: 28240-5. PMID: 10497179
2. Regulation of carbamoyl phosphate synthetase by MAP kinase.  |  Graves, LM., et al. 2000. Nature. 403: 328-32. PMID: 10659854
3. Localization of the multifunctional protein CAD in astrocytes of rodent brain.  |  Cammer, W. and Downing, M. 1991. J Histochem Cytochem. 39: 695-700. PMID: 1673139
4. Cloning and sequence of a cDNA encoding human carbamyl phosphate synthetase I: molecular analysis of hyperammonemia.  |  Haraguchi, Y., et al. 1991. Gene. 107: 335-40. PMID: 1840546
5. Phosphorylation and activation of hamster carbamyl phosphate synthetase II by cAMP-dependent protein kinase. A novel mechanism for regulation of pyrimidine nucleotide biosynthesis.  |  Carrey, EA., et al. 1985. EMBO J. 4: 3735-42. PMID: 4092695
6. Phosphorylation and dephosphorylation of carbamoyl-phosphate synthetase II complex of rat ascites hepatoma cells.  |  Otsuki, T., et al. 1981. J Biochem. 89: 1367-74. PMID: 6115855
7. Mice deficient in the urea-cycle enzyme, carbamoyl phosphate synthetase I, die during the early neonatal period from hyperammonemia.  |  Schofield, JP., et al. 1999. Hepatology. 29: 181-5. PMID: 9862865

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-CPS2 Antibody (F-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-p-CPS2 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-CPS2 Antibody (F-4) Specifications

Alternate Product Name: CPS2

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Thr 456

Epitope Target: CPS2

Epitope Species: human

Product Reactivity/Detection: Thr 456 phosphorylated CPS2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for p-CPS2 Antibody (F-4): P: Blocking Peptide

The preferred CPS2 antibody is CPS2 Antibody (F-6): sc-376072.