CPEB Antibody (A-9): sc-514683

CPEB Antibody (A-9) is a mouse monoclonal IgA antibody that detects CPEB in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CPEB, or cytoplasmic polyadenylation element binding protein, plays a crucial role in the regulation of messenger RNA translation, which is vital for various cellular processes including cell-cycle progression and early development. CPEB is primarily located in the cytoplasm, where CPEB binds to specific RNA sequences known as cytoplasmic polyadenylation elements, thereby influencing the stability and localization of mRNAs. CPEB′s ability to modulate translational repression is particularly important during oocyte maturation, as CPEB ensures the timely activation of mRNAs necessary for this process. Additionally, CPEB is synthesized during oogenesis and stored in the oocyte until needed, with degradation occurring via the proteasome pathway, often involving ubiquitin-conjugated intermediates. Anti-CPEB antibody (A-9) is an essential tool for researchers studying the intricate mechanisms of mRNA regulation and its implications in developmental biology and neurobiology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CPEB Antibody (A-9) References:

1. CPEB phosphorylation and cytoplasmic polyadenylation are catalyzed by the kinase IAK1/Eg2 in maturing mouse oocytes.  |  Hodgman, R., et al. 2001. Development. 128: 2815-22. PMID: 11526086
2. Differential mRNA translation and meiotic progression require Cdc2-mediated CPEB destruction.  |  Mendez, R., et al. 2002. EMBO J. 21: 1833-44. PMID: 11927567
3. A neuronal isoform of the aplysia CPEB has prion-like properties.  |  Si, K., et al. 2003. Cell. 115: 879-91. PMID: 14697205
4. Control of cellular senescence by CPEB.  |  Groisman, I., et al. 2006. Genes Dev. 20: 2701-12. PMID: 17015432
5. A molecular circuit composed of CPEB-1 and c-Jun controls growth hormone-mediated synaptic plasticity in the mouse hippocampus.  |  Zearfoss, NR., et al. 2008. J Neurosci. 28: 8502-9. PMID: 18716208
6. The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo.  |  Bestman, JE. and Cline, HT. 2009. Front Neural Circuits. 3: 10. PMID: 19753328
7. Protein-only mechanism induces self-perpetuating changes in the activity of neuronal Aplysia cytoplasmic polyadenylation element binding protein (CPEB).  |  Heinrich, SU. and Lindquist, S. 2011. Proc Natl Acad Sci U S A. 108: 2999-3004. PMID: 21270333
8. Characterization of prion-like conformational changes of the neuronal isoform of Aplysia CPEB.  |  Raveendra, BL., et al. 2013. Nat Struct Mol Biol. 20: 495-501. PMID: 23435382
9. The 3'UTR of the Drosophila CPEB translation factor gene orb2 plays a crucial role in spermatogenesis.  |  Gilmutdinov, R., et al. 2021. Development. 148: PMID: 34473243
10. Specificity of RNA binding by CPEB: requirement for RNA recognition motifs and a novel zinc finger.  |  Hake, LE., et al. 1998. Mol Cell Biol. 18: 685-93. PMID: 9447964

The preferred CPEB antibody is CPEB Antibody (G-6): sc-514688.