Cot Antibody (H-7): sc-373677

Cot Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cot protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Cot antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cot protein, also known as mitogen-activated protein kinase kinase kinase 8, plays a crucial role in cell signaling pathways, particularly in activating the mitogen-activated protein kinase (MAPK) pathway, which regulates cellular processes like proliferation, differentiation, and survival. Cot protein is primarily located in the cytoplasm, where Cot interacts with other signaling molecules to modulate cellular responses to external stimuli. Notably, Cot has been implicated in T lymphocyte activation, highlighting Cot′s importance in immune responses. Additionally, Cot protein undergoes post-translational modifications that influence activity and interactions, further underscoring Cot′s role in cellular signaling networks. Overexpression of Cot induces MAP kinase activation, and Cot′s function is intricately linked to proteins such as Ras and Raf-1, suggesting Cot may form a multimeric complex that regulates downstream signaling events. This complex interplay of interactions and modifications makes Cot a significant target for research in cancer biology and immunology.

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Cot Antibody (H-7) References:

1. Identification of a novel human kinase supporter of Ras (hKSR-2) that functions as a negative regulator of Cot (Tpl2) signaling.  |  Channavajhala, PL., et al. 2003. J Biol Chem. 278: 47089-97. PMID: 12975377
2. Cell biology. A signal chain of events.  |  Roberts, TM. 1992. Nature. 360: 534-5. PMID: 1334231
3. Ras p21: effects and regulation.  |  Haubruck, H. and McCormick, F. 1991. Biochim Biophys Acta. 1072: 215-29. PMID: 1751548
4. Cot/Tpl-2 protein kinase as a target for the treatment of inflammatory disease.  |  George, D. and Salmeron, A. 2009. Curr Top Med Chem. 9: 611-22. PMID: 19689369
5. COT drives resistance to RAF inhibition through MAP kinase pathway reactivation.  |  Johannessen, CM., et al. 2010. Nature. 468: 968-72. PMID: 21107320
6. Cot/tpl2 participates in the activation of macrophages by adiponectin.  |  Sanz-Garcia, C., et al. 2014. J Leukoc Biol. 95: 917-30. PMID: 24532642
7. Tumor progression locus 2 (TPL2): A Cot-plicated progression from inflammation to chronic liver disease.  |  Gutierrez, AH., et al. 2023. Biochim Biophys Acta Mol Basis Dis. 1869: 166660. PMID: 36764206
8. Tpl-2 acts in concert with Ras and Raf-1 to activate mitogen-activated protein kinase.  |  Patriotis, C., et al. 1994. Proc Natl Acad Sci U S A. 91: 9755-9. PMID: 7937886
9. The human cot proto-oncogene encodes two protein serine/threonine kinases with different transforming activities by alternative initiation of translation.  |  Aoki, M., et al. 1993. J Biol Chem. 268: 22723-32. PMID: 8226782
10. Requirement for Raf and MAP kinase function during the meiotic maturation of Xenopus oocytes.  |  Fabian, JR., et al. 1993. J Cell Biol. 122: 645-52. PMID: 8335690
11. The MAP kinase cascade is essential for diverse signal transduction pathways.  |  Nishida, E. and Gotoh, Y. 1993. Trends Biochem Sci. 18: 128-31. PMID: 8388132
12. Cot kinase activates tumor necrosis factor-alpha gene expression in a cyclosporin A-resistant manner.  |  Ballester, A., et al. 1998. J Biol Chem. 273: 14099-106. PMID: 9603908

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cot Antibody (H-7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Cot Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cot Antibody (H-7) Specifications

Alternate Product Name: MAP3K8

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 441-467

Epitope Target: Cot

Epitope Species: mouse

Product Reactivity/Detection: Cot

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Cot Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin