Cortactin Antibody (H-5): sc-55579
- Cortactin Antibody (H-5) is a mouse monoclonal IgG1 κ Cortactin antibody, cited in 23 publications, provided at 200 µg/ml
- raised against amino acids 309-499 of Cortactin of human origin
- Cortactin Antibody (H-5) is recommended for detection of Cortactin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Cortactin Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cortactin Antibody (H-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Cortactin Antibody (H-5) (see ordering information below).
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Cortactin Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect Cortactin, also known as CTTN or Ems-1, a crucial protein predominantly located in the cytoplasm where it regulates the actin cytoskeleton. Raised against amino acids 309-499 of human Cortactin, anti-Cortactin antibody (H-5) exhibits broad species reactivity, effectively recognizing Cortactin from mouse, rat, and human origins. Cortactin plays an essential role in various cellular processes, including cell motility, endocytosis, and maintaining cell shape, making Cortactin (H-5) antibody vital for studies on cellular architecture and dynamics. Cortactin monoclonal antibody (H-5) is versatile in applications, suitable for western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), thereby accommodating a wide range of experimental needs. Structurally, Cortactin contains tandem 37 amino acid repeats at the N-terminus and an SH3 domain at the C-terminus, which are critical for interaction with filamentous actin (F-actin) and various signaling molecules. Phosphorylation by Src p60 kinase regulates F-actin binding and cross-linking, modulating cytoskeletal functions and influencing cellular structural integrity. Additionally, Cortactin responds to growth factors such as FGF-1, highlighting involvement in signal transduction pathways. Abundant expression in megakaryocytes and platelets suggests a significant role in maturation and function of these cells. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, Cortactin (H-5) monoclonal antibody provides researchers with flexible options to suit diverse experimental requirements. Anti-Cortactin antibody (H-5) is an indispensable tool for scientists investigating cytoskeletal dynamics, cell signaling mechanisms, and hematopoietic processes, ensuring accurate and reliable detection in studies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cortactin Antibody (H-5) References:
- Association of fibroblast growth factor receptor-1 with c-Src correlates with association between c-Src and cortactin. | Zhan, X., et al. 1994. J Biol Chem. 269: 20221-4. PMID: 7519605
- p80/85 cortactin associates with the Src SH2 domain and colocalizes with v-Src in transformed cells. | Okamura, H. and Resh, MD. 1995. J Biol Chem. 270: 26613-8. PMID: 7592885
- Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous actin-binding protein enriched in the cell cortex. | Wu, H. and Parsons, JT. 1993. J Cell Biol. 120: 1417-26. PMID: 7680654
- Murine cortactin is phosphorylated in response to fibroblast growth factor-1 on tyrosine residues late in the G1 phase of the BALB/c 3T3 cell cycle. | Zhan, X., et al. 1993. J Biol Chem. 268: 24427-31. PMID: 7693700
- Upregulation of cortactin expression during the maturation of megakaryocytes. | Zhan, X., et al. 1997. Blood. 89: 457-64. PMID: 9002947
- Down-regulation of the filamentous actin cross-linking activity of cortactin by Src-mediated tyrosine phosphorylation. | Huang, C., et al. 1997. J Biol Chem. 272: 13911-5. PMID: 9153252
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cortactin Antibody (H-5) | sc-55579 | 200 µg/ml | $316.00 | |||
Cortactin Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-528604 | 200 µg Ab; 10 µg BP | $354.00 | |||
Cortactin Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-521024 | 200 µg Ab, 40 µg BP | $354.00 | |||
Cortactin Antibody (H-5): m-IgG1 BP-HRP Bundle | sc-543023 | 200 µg Ab; 20 µg BP | $354.00 | |||
Cortactin Antibody (H-5) AC | sc-55579 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Cortactin Antibody (H-5) HRP | sc-55579 HRP | 200 µg/ml | $316.00 | |||
Cortactin Antibody (H-5) FITC | sc-55579 FITC | 200 µg/ml | $330.00 | |||
Cortactin Antibody (H-5) PE | sc-55579 PE | 200 µg/ml | $343.00 | |||
Cortactin Antibody (H-5) Alexa Fluor® 488 | sc-55579 AF488 | 200 µg/ml | $357.00 | |||
Cortactin Antibody (H-5) Alexa Fluor® 546 | sc-55579 AF546 | 200 µg/ml | $357.00 | |||
Cortactin Antibody (H-5) Alexa Fluor® 594 | sc-55579 AF594 | 200 µg/ml | $357.00 | |||
Cortactin Antibody (H-5) Alexa Fluor® 647 | sc-55579 AF647 | 200 µg/ml | $357.00 | |||
Cortactin Antibody (H-5) Alexa Fluor® 680 | sc-55579 AF680 | 200 µg/ml | $357.00 | |||
Cortactin Antibody (H-5) Alexa Fluor® 790 | sc-55579 AF790 | 200 µg/ml | $357.00 |