Date published: 2025-10-19

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COPB Antibody (D-9): sc-165975

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Datasheets
  • COPB Antibody (D-9) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 654-953 mapping at the C-terminus of COPB of human origin
  • recommended for detection of COPB of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for COPB Antibody (D-9) for WB applications. These reagents are now offered in bundles with COPB Antibody (D-9) (see ordering information below).

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COPB Antibody (D-9) is a mouse monoclonal IgG1 antibody that detects COPB in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). COPB, also known as β-COP, plays a crucial role in membrane and vesicular trafficking within the early secretory pathway, specifically as a marker protein for pre-Golgi intermediates, including vesicular tubular clusters (VTCs). COPB functions as a major component of the coat of non-Clathrin-coated vesicles, which are essential for mediating membrane traffic through the Golgi complex. This trafficking is vital for proper sorting and delivery of proteins and lipids, ensuring cellular processes function efficiently. Additionally, COPB interacts with various proteins, such as GIV (Gα i/s interacting protein) and β′-COP (COPP), which further underscores COPB′s significance in dynamic regulation of vesicular transport. Anti-COPB antibody (D-9) serves as an invaluable tool for researchers studying cellular transport mechanisms and their implications in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

COPB Antibody (D-9)

sc-165975
200 µg/ml
$316.00

COPB Antibody (D-9): m-IgG Fc BP-HRP Bundle

sc-537426
200 µg Ab; 10 µg BP
$354.00

COPB Antibody (D-9): m-IgGκ BP-HRP Bundle

sc-534684
200 µg Ab; 40 µg BP
$354.00

What is the recommended starting dilution for Western blot for COPB (D-9): sc-165975 monoclonal antibody?

Asked by: Cweed
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-165975.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Produced positive Western Blot data of COPBProduced positive Western Blot data of COPB expression in non-transfected and mouse COPB transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Great Western Blot data of COPB expressionGreat Western Blot data of COPB expression in Hep G2, C6 and HeLa whole cell lysates. -SCBT QC
Date published: 2014-08-04
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COPB Antibody (D-9) is rated 5.0 out of 5 by 2.
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