COPB Antibody (D-10): sc-393615
- COPB Antibody (D-10) is a mouse monoclonal IgG2b κ COPB antibody, cited in 14 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-19 at the N-terminus of COPB of rat origin
- COPB Antibody (D-10) is recommended for detection of COPB of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-COPB Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for COPB Antibody (D-10) for WB and IHC(P) applications. These reagents are now offered in bundles with COPB Antibody (D-10) (see ordering information below).
COPB Antibody (D-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects COPB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). COPB (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. COPB protein, also known as β-COP, plays a crucial role in membrane and vesicular trafficking within the early secretory pathway, specifically mediating protein transport from the endoplasmic reticulum to the Golgi apparatus through non-Clathrin-coated vesicles. COPB functions in maintaining cellular homeostasis and ensures proteins are correctly processed and delivered to their destinations. COPB serves as a marker for pre-Golgi intermediates, such as vesicular tubular clusters (VTCs), which are essential for cargo organization and transport within cells. COPB interacts with GIV (Gα i/s interacting protein), co-localizing with Gα i3 on vesicles near endoplasmic reticulum exit sites and cis Golgi cisternae, highlighting COPB′s importance in vesicular transport regulation. COPB′s interaction with Afadin DIL domain-interacting protein (ADIP) at the Golgi complex underscores COPB′s role in coordinating trafficking processes necessary for proper cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
COPB Antibody (D-10) References:
- Atypical protein kinase C plays a critical role in protein transport from pre-Golgi intermediates. | Tisdale, EJ., et al. 2003. J Biol Chem. 278: 38015-21. PMID: 12871960
- Deubiquitination, a new player in Golgi to endoplasmic reticulum retrograde transport. | Cohen, M., et al. 2003. J Biol Chem. 278: 51989-92. PMID: 14593109
- Involvement of beta-COP in membrane traffic through the Golgi complex. | Duden, R., et al. 1991. Trends Cell Biol. 1: 14-9. PMID: 14731804
- Coronin 7, the mammalian POD-1 homologue, localizes to the Golgi apparatus. | Rybakin, V., et al. 2004. FEBS Lett. 573: 161-7. PMID: 15327992
- Afadin- and alpha-actinin-binding protein ADIP directly binds beta'-COP, a subunit of the coatomer complex. | Asada, M., et al. 2004. Biochem Biophys Res Commun. 321: 350-4. PMID: 15358183
- Identification and characterization of GIV, a novel Galpha i/s-interacting protein found on COPI, endoplasmic reticulum-Golgi transport vesicles. | Le-Niculescu, H., et al. 2005. J Biol Chem. 280: 22012-20. PMID: 15749703
- In vitro assembly and disassembly of coatomer. | Lowe, M. and Kreis, TE. 1995. J Biol Chem. 270: 31364-71. PMID: 8537409
- A single binding site for dilysine retrieval motifs and p23 within the gamma subunit of coatomer. | Harter, C. and Wieland, FT. 1998. Proc Natl Acad Sci U S A. 95: 11649-54. PMID: 9751720
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COPB Antibody (D-10) | sc-393615 | 200 µg/ml | $316.00 | |||
COPB Antibody (D-10): m-IgGκ BP-HRP Bundle | sc-524025 | 200 µg Ab, 40 µg BP | $354.00 | |||
COPB Antibody (D-10): m-IgG2b BP-HRP Bundle | sc-549150 | 200 µg Ab; 10 µg BP | $354.00 | |||
COPB Antibody (D-10) AC | sc-393615 AC | 500 µg/ml, 25% agarose | $416.00 | |||
COPB Antibody (D-10) HRP | sc-393615 HRP | 200 µg/ml | $316.00 | |||
COPB Antibody (D-10) FITC | sc-393615 FITC | 200 µg/ml | $330.00 | |||
COPB Antibody (D-10) PE | sc-393615 PE | 200 µg/ml | $343.00 | |||
COPB Antibody (D-10) Alexa Fluor® 488 | sc-393615 AF488 | 200 µg/ml | $357.00 | |||
COPB Antibody (D-10) Alexa Fluor® 546 | sc-393615 AF546 | 200 µg/ml | $357.00 | |||
COPB Antibody (D-10) Alexa Fluor® 594 | sc-393615 AF594 | 200 µg/ml | $357.00 | |||
COPB Antibody (D-10) Alexa Fluor® 647 | sc-393615 AF647 | 200 µg/ml | $357.00 | |||
COPB Antibody (D-10) Alexa Fluor® 680 | sc-393615 AF680 | 200 µg/ml | $357.00 | |||
COPB Antibody (D-10) Alexa Fluor® 790 | sc-393615 AF790 | 200 µg/ml | $357.00 | |||
COPB (D-10) Neutralizing Peptide | sc-393615 P | 100 µg/0.5 ml | $68.00 |