COL1A2 Antibody (H-9) recommended for detection of Collagen α2 Type I of mouse, rat and human origin by WB, IP, IF and ELISA

COL1A2 Antibody (H-9): sc-376350

COL1A2 Antibody (H-9) is rated 3.5 out of 5 by 6.
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Datasheets

    • COL1A2 Antibody (H-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1021-1090 mapping within an internal region of Collagen α2 Type I of human origin
    • Anti-COL1A2 Antibody (H-9) is recommended for detection of Collagen α2 Type I of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • Anti-COL1A2 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of COL1A2 (H-9): sc-376350.
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Are there any issues with using sc-376350: COL1A2 (H-9) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-376350 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 1 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 1 out of 5 by from 有很严重的问题 我们实验室之前用过的抗体Col1a2,可是我western blot实验中在70kd的位置只有很浅的条带,怎么办?
Date published: 2018-05-17
Rated 5 out of 5 by from It works for western blotting! It detected endogenous COL1A2 in immortalized HFFs (1:500 in 5% milk/TBS-Tween, overnight at 4C).
Date published: 2017-11-28
Rated 5 out of 5 by from Great for WB This antibody is good for COL1A2 in WB. Strong and clear band observed in WB of adipose tissue of mouse.
Date published: 2017-10-20
Rated 5 out of 5 by from Good choice for Western Blot This antibody shows good detection of COL1A2 at the high molecular weight with a variety of whole cell lysates.
Date published: 2017-02-07
Rated 4 out of 5 by from Worked well in Western blot applications Worked well in Western blot applications with CCD1064Sk. -SCBT QC
Date published: 2015-04-11
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